Overview

  • Product name

  • Description

    Rabbit polyclonal to ALDH1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Chimpanzee
  • Immunogen

    Recombinant fragment within Human ALDH1A1 (internal sequence). The exact sequence is proprietary.
    Database link: P00352

  • Positive control

    • WB: A549 whole cell lysate; Mouse and rat liver lysates. IHC-P: Human gastric cancer tissue. ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 55 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • Pathway

    Cofactor metabolism; retinol metabolism.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetaldehyde dehydrogenase 1 antibody
    • AHD2 antibody
    • AL1A1_HUMAN antibody
    • ALDC antibody
    • Aldehyde dehydrogenase 1 family member A1 antibody
    • Aldehyde dehydrogenase 1 soluble antibody
    • Aldehyde dehydrogenase 1A1 antibody
    • Aldehyde dehydrogenase antibody
    • Aldehyde dehydrogenase cytosolic antibody
    • Aldehyde dehydrogenase family 1 member A1 antibody
    • Aldehyde dehydrogenase liver cytosolic antibody
    • ALDH 1 antibody
    • ALDH 1A1 antibody
    • ALDH class 1 antibody
    • ALDH, liver cytosolic antibody
    • ALDH-E1 antibody
    • ALDH1 A1 antibody
    • ALDH1 antibody
    • ALDH11 antibody
    • ALDH1A1 antibody
    • ALHDII antibody
    • cytosolic antibody
    • epididymis luminal protein 12 antibody
    • epididymis luminal protein 9 antibody
    • epididymis secretory sperm binding protein Li 53e antibody
    • HEL-S-53e antibody
    • MGC2318 antibody
    • PUMB1 antibody
    • RALDH 1 antibody
    • RalDH1 antibody
    • Retinal dehydrogenase 1 antibody
    see all

Images

  • Paraffin-embedded human gastric cancer tissue stained for ALDH1A1 using ab227964 at 1/500 dilution in immunohistochemical analysis.

  • Anti-ALDH1A1 antibody (ab227964) at 1/10000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 55 kDa



    7.5% SDS-PAGE gel.

  • Paraformaldehyde-fixed A549 (human lung carcinoma cell line) cells stained for ALDH1A1 (green) using ab227964 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

  • Anti-ALDH1A1 antibody (ab227964) at 1/5000 dilution + Mouse liver lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

  • Anti-ALDH1A1 antibody (ab227964) at 1/5000 dilution + Rat liver lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

References

ab227964 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227964.
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