Anti-ALDH1A1 antibody (ab9883)
- Datasheet
- References (11)
- Protocols
Overview
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Product name
Anti-ALDH1A1 antibody
See all ALDH1A1 primary antibodies -
Description
Goat polyclonal to ALDH1A1 -
Host species
Goat -
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2). -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.
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Positive control
- Human liver lysates.
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General notes
A protease inhibitor that inhibits fibrin clot formation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
A protease inhibitor that inhibits fibrin clot formation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 18560594 | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa. 1 hour primary incubation is recommended for this product. |
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| IHC-P | Use a concentration of 5 µg/ml. |
Application notes
Is unsuitable for ICC.
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281615 Cow
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P48644 Cow
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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Western blot - Anti-ALDH1A1 antibody (ab9883)Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 55 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-ALDH1A1 antibody (ab9883)This image is courtesy of an anonymous AbreviewAnti-ALDH1A1 antibody (ab9883) at 1/500 dilution + MDBK cell lysate at 100 µg
Secondary
HRP-conjugated Donkey anti-goat IgG
Predicted band size: 55 kDa
Datasheets and documents
References
This product has been referenced in:
- Wang R et al. Quercetin Inhibits Breast Cancer Stem Cells via Downregulation of Aldehyde Dehydrogenase 1A1 (ALDH1A1), Chemokine Receptor Type 4 (CXCR4), Mucin 1 (MUC1), and Epithelial Cell Adhesion Molecule (EpCAM). Med Sci Monit 24:412-420 (2018). Read more (PubMed: 29353288) »
- Goto S et al. Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife 7:N/A (2018). IHC-P ; Mouse . Read more (PubMed: 29609731) »
