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Anti-ALDH1A1 antibody (ab9883)

  • Datasheet
  • SDS
Reviews (4)Q&A (13)References (12)

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Western blot - Anti-ALDH1A1 antibody (ab9883)
  • Western blot - Anti-ALDH1A1 antibody (ab9883)

Key features and details

  • Goat polyclonal to ALDH1A1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ALDH1A1 antibody
    See all ALDH1A1 primary antibodies
  • Description

    Goat polyclonal to ALDH1A1
  • Host species

    Goat
  • Specificity

    This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design, and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa).

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes


    A protease inhibitor that inhibits fibrin clot formation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes

    A protease inhibitor that inhibits fibrin clot formation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human ALDH1A1 protein (ab173068)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.

1 hour primary incubation is recommended for this product.

Notes
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.

1 hour primary incubation is recommended for this product.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
    • Pathway

      Cofactor metabolism; retinol metabolism.
    • Sequence similarities

      Belongs to the aldehyde dehydrogenase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P00352 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 281615 Cow
      • Entrez Gene: 216 Human
      • Entrez Gene: 11668 Mouse
      • Entrez Gene: 24188 Rat
      • Omim: 100640 Human
      • SwissProt: P48644 Cow
      • SwissProt: P00352 Human
      • SwissProt: P24549 Mouse
      • SwissProt: P51647 Rat
      • Unigene: 76392 Human
      • Unigene: 250866 Mouse
      • Unigene: 6132 Rat
      see all
    • Alternative names

      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Images

    • Western blot - Anti-ALDH1A1 antibody (ab9883)
      Western blot - Anti-ALDH1A1 antibody (ab9883)
      All lanes : Anti-ALDH1A1 antibody (ab9883) at 0.3 µg/ml

      Lane 1 : Human liver lysate (protein in RIPA buffer)
      Lane 2 : Rat liver lysate (protein in RIPA buffer)
      Lane 3 : Mouse liver lysate (protein in RIPA buffer)

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 55 kDa



      Detected by chemiluminescence.

    • Western blot - Anti-ALDH1A1 antibody (ab9883)
      Western blot - Anti-ALDH1A1 antibody (ab9883)This image is courtesy of an anonymous Abreview
      Anti-ALDH1A1 antibody (ab9883) at 1/500 dilution + MDBK cell lysate at 100 µg

      Secondary
      HRP-conjugated Donkey anti-goat IgG

      Predicted band size: 55 kDa

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (12)

    Publishing research using ab9883? Please let us know so that we can cite the reference in this datasheet.

    ab9883 has been referenced in 12 publications.

    • Flori L  et al. Development of Fortified Citrus Olive Oils: From Their Production to Their Nutraceutical Properties on the Cardiovascular System. Nutrients 12:N/A (2020). PubMed: 32471156
    • Wang R  et al. Quercetin Inhibits Breast Cancer Stem Cells via Downregulation of Aldehyde Dehydrogenase 1A1 (ALDH1A1), Chemokine Receptor Type 4 (CXCR4), Mucin 1 (MUC1), and Epithelial Cell Adhesion Molecule (EpCAM). Med Sci Monit 24:412-420 (2018). PubMed: 29353288
    • Goto S  et al. Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife 7:N/A (2018). IHC-P ; Mouse . PubMed: 29609731
    • Qin J  et al. Tanshinone IIA inhibits cervix carcinoma stem cells migration and invasion via inhibiting YAP transcriptional activity. Biomed Pharmacother 105:758-765 (2018). PubMed: 29909343
    • Hu Z  et al. Transient receptor potential vanilloid-type 2 targeting on stemness in liver cancer. Biomed Pharmacother 105:697-706 (2018). PubMed: 29906748
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-ALDH1A1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pleurodeles waltl Tissue sections (brain)
    Permeabilization
    Yes - 0.25% Triton-X
    Specification
    brain
    Blocking step
    fish skin gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2020

    Immunohistochemistry (Resin sections) abreview for Anti-ALDH1A1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Rabbit Tissue sections (Cornea)
    Specification
    Cornea
    Read More

    Julia Fernandez

    Verified customer

    Submitted Nov 28 2019

    Western blot abreview for Anti-ALDH1A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDBK cells)
    Loading amount
    100 µg
    Specification
    MDBK cells
    Gel Running Conditions
    Reduced Denaturing (Tris-Glycine gel - 10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 08 2013

    Western blot abreview for Anti-ALDH1A1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - other (MDCK cell lysate)
    Loading amount
    100 µg
    Specification
    MDCK cell lysate
    Gel Running Conditions
    Reduced Denaturing (Tris-Glycine gel - 10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 05 2013

    Question

    We’ll take the goat polyclonal ab115252.  Can you send us the protocol that you recommend for this antibody? 

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2011

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab115252. This product is currently out of stock and delivery is expected around November 9. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2011

    Question

    It would be best for us is we can get a replacement for ab9883. Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2011

    Answer

    Thank you for your reply. As we have already sent a vial of ab9883 as a replacement and neither ab9883 vial has produced acceptable results, we generally would send a different antibody to try. We also have a mouse monoclonal ab105920, and a different goat polyclonal ab115252 that have been tested in human IHC-P:  https://www.abcam.com/ALDH1A1-antibody-5A11-ab105920.html https://www.abcam.com/ALDH1A1-antibody-ab115252.html Please let me know if one of these is acceptable and I will send it to you promptly.  

    Read More

    Abcam Scientific Support

    Answered on Nov 01 2011

    Question

    I’m having trouble finding the PO number.  I know the original order was placed around 2/16/11.  We changed which staff was taking care of orders at the end of February so I’m not sure if I can find the PO number. We must use the antibody whose host is not rabbit since later we will try to do double staining and the other antibody we use is rabbit antibody. Therefore we can not use the ab23375. Thanks   

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Thank you for your reply. I think we have found the original order from February, but as we have sent a replacement vial of ab9883 which also did not work, in this case it might be best to issue a credit note or refund since the rabbit polyclonal ab23375 can not be used. Please let me know if this is an acceptable solution and I will issue the credit/refund immediately. Let me know if you have any questions or if there is anything else we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2011

    Question

    Can you send us the same one that we were trying to use (ab9883)?  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2011

    Answer

    Thank you for your reply.  Could you please provide your original order or PO number?  I did find an order that went to your institution,  however we had already sent a replacement vial of ab9883 on that order.  If you have not been able to obtain satisfactory results with two vials of ab9883, an alternative antibody may work better in your application.  As Caitlin had mentioned, ab23375 is highly rated and would also be guaranteed for your experiment.  Alternatively, I would be happy to offer you a credit or refund.  Please let me know your original order or PO number and how you would like to proceed and I am happy to assist you further. 

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2011

    Question

    What is the host animal of the other antibody?  Does it work with paraffin embedded sections?  What is the cross reactivity?  We’ll decide which one we want based on these questions.  Thank you  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 20 2011

    Answer

    Thanks for your reply. We have a few other ALDH1A1 antibodies but this is our most popular and has very good reviews/publications. It is a rabbit polyclonal, it does work in paraffin sections, and it has been tested for cross-reactivity with human, mouse, rat, dog, goat, and sheep samples. Please let me know if you have any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 20 2011

    Question

     It’s still not working for us.  We tried it with dilutions of 1:50, 1:75, and 1:00.  Can you send us a new antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 20 2011

    Answer

    Thanks for keeping me updated. I would be happy to send a replacement antibody. Do you want another vial of ab9883 or would you prefer to try a different ALDH1A1 antibody? We also have ab23375 which has been used in 5 references and has a 4-star rating from other users- https://www.abcam.com/ALDH1A1-antibody-Neuronal-Marker-ab23375.html Please let me know which you would prefer. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Oct 20 2011

    1-10 of 17 Abreviews or Q&A

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