Overview

  • Product name
    Anti-ALDH1A1 antibody
    See all ALDH1A1 primary antibodies
  • Description
    Goat polyclonal to ALDH1A1
  • Host species
    Goat
  • Specificity
    This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human liver lysates.
  • General notes


    A protease inhibitor that inhibits fibrin clot formation.

Properties

Applications

Our Abpromise guarantee covers the use of ab9883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18560594
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.Can be blocked with Human ALDH1A1 peptide (ab23169).
IHC-P Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function
      Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
    • Pathway
      Cofactor metabolism; retinol metabolism.
    • Sequence similarities
      Belongs to the aldehyde dehydrogenase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Images

    • Western blot - Anti-ALDH1A1 antibody (ab9883)
      Western blot - Anti-ALDH1A1 antibody (ab9883)
      Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size: 55 kDa



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)
      IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Western blot - Anti-ALDH1A1 antibody (ab9883)
      Western blot - Anti-ALDH1A1 antibody (ab9883)This image is courtesy of an anonymous Abreview
      Anti-ALDH1A1 antibody (ab9883) at 1/500 dilution + MDBK cell lysate at 100 µg

      Secondary
      HRP-conjugated Donkey anti-goat IgG

      Predicted band size: 55 kDa

      See Abreview

    References

    This product has been referenced in:
    • Wang R  et al. Quercetin Inhibits Breast Cancer Stem Cells via Downregulation of Aldehyde Dehydrogenase 1A1 (ALDH1A1), Chemokine Receptor Type 4 (CXCR4), Mucin 1 (MUC1), and Epithelial Cell Adhesion Molecule (EpCAM). Med Sci Monit 24:412-420 (2018). Read more (PubMed: 29353288) »
    • Goto S  et al. Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife 7:N/A (2018). IHC-P ; Mouse . Read more (PubMed: 29609731) »

    See all 7 Publications for this product

    Publishing research using ab9883? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    15 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ALDH1A1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDBK cells)
    Loading amount
    100 µg
    Specification
    MDBK cells
    Gel Running Conditions
    Reduced Denaturing (Tris-Glycine gel - 10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 08 2013

    Western blot abreview for Anti-ALDH1A1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - other (MDCK cell lysate)
    Loading amount
    100 µg
    Specification
    MDCK cell lysate
    Gel Running Conditions
    Reduced Denaturing (Tris-Glycine gel - 10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Feb 05 2013

    We’ll take the goat polyclonal ab115252.  Can you send us the protocol that you recommend for this antibody?

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab115252. This product is currently out of stock and delivery is expe...

    Read More

    It would be best for us is we can get a replacement for ab9883. Thank you,

    Thank you for your reply. As we have already sent a vial of ab9883 as a replacement and neither ab9883 vial has produced acceptable results, we generally would send a different antibody to try. We also have a mouse monoclonal ab105920, and a different ...

    Read More

    I’m having trouble finding the PO number.  I know the original order was placed around 2/16/11.  We changed which staff was taking care of orders at the end of February so I’m not sure if I can find the PO number. We must use the antibody whose host is...

    Read More

    Thank you for your reply. I think we have found the original order from February, but as we have sent a replacement vial of ab9883 which also did not work, in this case it might be best to issue a credit note or refund since the rabbit polyclonal ab233...

    Read More

    Can you send us the same one that we were trying to use (ab9883)?

    Thank you for your reply.  Could you please provide your original order or PO number?  I did find an order that went to your institution,  however we had already sent a replacement vial of ab9883 on that order.  If you have not been able to obtain sati...

    Read More

    What is the host animal of the other antibody?  Does it work with paraffin embedded sections?  What is the cross reactivity?  We’ll decide which one we want based on these questions.  Thank you

    Thanks for your reply. We have a few other ALDH1A1 antibodies but this is our most popular and has very good reviews/publications. It is a rabbit polyclonal, it does work in paraffin sections, and it has been tested for cross-reactivity with hu...

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    It’s still not working for us.  We tried it with dilutions of 1:50, 1:75, and 1:00.  Can you send us a new antibody?

    Thanks for keeping me updated. I would be happy to send a replacement antibody. Do you want another vial of ab9883 or would you prefer to try a different ALDH1A1 antibody? We also have ab23375 which has been used in 5 references and has a 4-star rating...

    Read More

    I’ve pasted the answers to your questions into the email you sent me below. Thanks, Cory

    Thanks for your reply and for sending the answers to these questions. Your protocol seems very solid and the only thing I would recommend trying is to use a higher concentration of the primary antibody. Ab9883 is provided half as concentrated a...

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    We have been trying to get  you Goat pAb to ALDH1A1 ab9883 to work with Biocare Medicals Fast Red kit.  I’ve attached a copy of the protocol that we’ve been using.  We are having trouble getting this to work.  The slides we have been using work with th...

    Read More

    Thank you for contacting us and letting us know about the trouble with ab9883. I have looked through the protocol that you sent but I do have a few additional questions so that I can better understand the situation: 1) What was the Abcam order number o...

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