Anti-ALDH1A1 antibody (ab9883)
Key features and details
- Goat polyclonal to ALDH1A1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH1A1 antibody
See all ALDH1A1 primary antibodies -
Description
Goat polyclonal to ALDH1A1 -
Host species
Goat -
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design, and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa).
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.
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General notes
A protease inhibitor that inhibits fibrin clot formation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
A protease inhibitor that inhibits fibrin clot formation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281615 Cow
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P48644 Cow
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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Western blot - Anti-ALDH1A1 antibody (ab9883)All lanes : Anti-ALDH1A1 antibody (ab9883) at 0.3 µg/ml
Lane 1 : Human liver lysate (protein in RIPA buffer)
Lane 2 : Rat liver lysate (protein in RIPA buffer)
Lane 3 : Mouse liver lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 55 kDaDetected by chemiluminescence.
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Western blot - Anti-ALDH1A1 antibody (ab9883)This image is courtesy of an anonymous AbreviewAnti-ALDH1A1 antibody (ab9883) at 1/500 dilution + MDBK cell lysate at 100 µg
Secondary
HRP-conjugated Donkey anti-goat IgG
Predicted band size: 55 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab9883 has been referenced in 12 publications.
- Flori L et al. Development of Fortified Citrus Olive Oils: From Their Production to Their Nutraceutical Properties on the Cardiovascular System. Nutrients 12:N/A (2020). PubMed: 32471156
- Wang R et al. Quercetin Inhibits Breast Cancer Stem Cells via Downregulation of Aldehyde Dehydrogenase 1A1 (ALDH1A1), Chemokine Receptor Type 4 (CXCR4), Mucin 1 (MUC1), and Epithelial Cell Adhesion Molecule (EpCAM). Med Sci Monit 24:412-420 (2018). PubMed: 29353288
- Goto S et al. Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife 7:N/A (2018). IHC-P ; Mouse . PubMed: 29609731
- Qin J et al. Tanshinone IIA inhibits cervix carcinoma stem cells migration and invasion via inhibiting YAP transcriptional activity. Biomed Pharmacother 105:758-765 (2018). PubMed: 29909343
- Hu Z et al. Transient receptor potential vanilloid-type 2 targeting on stemness in liver cancer. Biomed Pharmacother 105:697-706 (2018). PubMed: 29906748