Overview

  • Product name
  • Description
    Goat polyclonal to ALDH1A1
  • Host species
    Goat
  • Specificity
    This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.

  • Positive control
    • Human liver lysates.
  • General notes


    A protease inhibitor that inhibits fibrin clot formation.

Properties

Applications

Our Abpromise guarantee covers the use of ab9883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18560594
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.Can be blocked with Human ALDH1A1 peptide (ab23169).
IHC-P Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
    • Pathway
      Cofactor metabolism; retinol metabolism.
    • Sequence similarities
      Belongs to the aldehyde dehydrogenase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Images

    • Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size: 55 kDa



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Anti-ALDH1A1 antibody (ab9883) at 1/500 dilution + MDBK cell lysate at 100 µg

      Secondary
      HRP-conjugated Donkey anti-goat IgG

      Predicted band size: 55 kDa

      See Abreview

    References

    This product has been referenced in:
    • Wang R  et al. Quercetin Inhibits Breast Cancer Stem Cells via Downregulation of Aldehyde Dehydrogenase 1A1 (ALDH1A1), Chemokine Receptor Type 4 (CXCR4), Mucin 1 (MUC1), and Epithelial Cell Adhesion Molecule (EpCAM). Med Sci Monit 24:412-420 (2018). Read more (PubMed: 29353288) »
    • Goto S  et al. Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells. Elife 7:N/A (2018). IHC-P ; Mouse . Read more (PubMed: 29609731) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    11-15 of 15 Abreviews or Q&A

    Answer

    Thank you for your enquiry. I have spoken to the Business Development Associate responsible for the Stem Cells core focus area and I have the following comments regarding its classification under the "hematopoietic stem cell" branch of our product browser. Ab9883 is classified as a ALDH1A1 antibody. However, on the datasheet it says it also cross-reacts with ALDH1A2 and ALDH1A3. I could find no mention of ALDH1A2 being a HSC marker but ALDH activity has been used to purify hematopoietic progenitors, e.g. Hess et al., 2004 http://www.bloodjournal.org/cgi/content/full/104/6/1648 Additionally, a quote from Forsberg et al. and Weissman (one of the world’s leading authorities on HSCs), 2005: “Aldehyde dehydrogenase, Aldh1a1, and Tek transcript levels are also higher in LT-HSC” (where LT stands for Long Term). http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1200425 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    I am afraid I don't know enough about this enzyme to answer your question. However the epitope used to generate the antibody is given on the datasheet - so if you know the sequences of different forms of Aldehyde Dehydrogenase you can check whether they are likely to be recognised by the antibody.

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    Answer

    Thank you for your enquiry. We are only able to provide the antibody in the 100ug size. To our knowledge, cross reactivity with rabbit has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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    Answer

    All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). According to the datasheet, this antibody has not been tested for immunohistochemistry.

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    Answer

    Unfortunately, we do not operate such a policy. The chances of this antibody reacting with rat are good, due to mice and rats being close species. However, since this antibody has not been tested on rat we can't guarantee success. You can contact abcam on: +44 (0)1223 472030

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    11-15 of 15 Abreviews or Q&A

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