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We have been trying to get you Goat pAb to ALDH1A1 ab9883 to work with Biocare Medicals Fast Red kit. I’ve attached a copy of the protocol that we’ve been using. We are having trouble getting this to work. The slides we have been using work with the rabbit antibody but we’re having trouble with the goat antibody. With the rabbit antibody we used the Mouse HRP-Polymer. Do you have any recommendations? Any help is greatly appreciated. Thanks,
Asked on Oct 14 2011
Thank you for contacting us and letting us know about the trouble with ab9883. I have looked through the protocol that you sent but I do have a few additional questions so that I can better understand the situation: 1) What was the Abcam order number or lot number of ab9883 that was used? 2) What kind of samples are being stained (e.g. what species and tissue type)? 3) What is the nature of the problem? Is there no staining, high background, or incorrect localization? 4) Was a 1:1000 dilution of the primary antibody used? We would recommend diluting the antibody 1:75 for use in IHC on paraffin-embedded tissue. 5) Is the rabbit antibody also a ALDH1A1 antibody? 6) Has the detection kit worked well with other goat primaries? We do guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase, so we would be happy to send a replacement or issue a credit or refund if the antibody is not working as expected. I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Oct 14 2011