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I’ve pasted the answers to your questions into the email you sent me below. Thanks, Cory
Asked on Oct 14 2011
Thanks for your reply and for sending the answers to these questions. Your protocol seems very solid and the only thing I would recommend trying is to use a higher concentration of the primary antibody. Ab9883 is provided half as concentrated as most of our other antibodies (0.5 mg/mL versus 1 mg/mL) and we do recommend a 1:75 dilution as stated previously. If there is still no staining after using this dilution, I can send a different antibody to try or alternatively issue a credit or refund. I look forward to hearing from you. Please keep me updated, and let me know if you have any questions.
Answered on Oct 15 2011