Western blot abreview for Anti-ALDH1A1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDBK cells)
Loading amount
100 µg
Specification
MDBK cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine gel - 10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T - 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-goat IgG - HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Positive control
MDBK cell lysate

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Submitted Feb 08 2013

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