Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1933Y] to ALDH1A1 (Alexa Fluor® 488)
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Product nameAnti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488)
See all ALDH1A1 primary antibodies
DescriptionRabbit monoclonal [EP1933Y] to ALDH1A1 (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
- ICC/IF: MCF7
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) (ab195255)
- Anti-ALDH1A1 antibody [EP1933Y] (HRP) (ab195517)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 594) (ab206884)
- Anti-ALDH1A1 antibody [EP1933Y] (PE) (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
- Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Our Abpromise guarantee covers the use of ab195254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
PathwayCofactor metabolism; retinol metabolism.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
- Information by UniProt
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab195254 has not yet been referenced specifically in any publications.