
Recombinant Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) (ab195255)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647)
See all ALDH1A1 primary antibodies -
Description
Rabbit monoclonal [EP1933Y] to ALDH1A1 (Alexa Fluor® 647) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- ICC/IF: MCF7 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP1933Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488) (ab195254)
- Anti-ALDH1A1 antibody [EP1933Y] (HRP) (ab195517)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 594) (ab206884)
- Anti-ALDH1A1 antibody [EP1933Y] (Phycoerythrin) (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
- Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab195255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/50. |
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- Unigene: 76392 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) (ab195255)
ab195255 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195255 at a working dilution of 1 in 50 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Datasheets and documents
References
This product has been referenced in:
- Colacino JA et al. Heterogeneity of Human Breast Stem and Progenitor Cells as Revealed by Transcriptional Profiling. Stem Cell Reports 10:1596-1609 (2018). Read more (PubMed: 29606612) »
- Chen Z et al. Aspirin cooperates with p300 to activate the acetylation of H3K9 and promote FasL-mediated apoptosis of cancer stem-like cells in colorectal cancer. Theranostics 8:4447-4461 (2018). Read more (PubMed: 30214631) »