Product nameAnti-ALDH1A1 antibody [EP1933Y] - C-terminal
See all ALDH1A1 primary antibodies
DescriptionRabbit monoclonal [EP1933Y] to ALDH1A1 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Does not react with: Rat
Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
- WB: HepG2 and NIH/3T3 cell lysate; Human small intestine and mouse liver tissue lysate. IHC: Human liver, kidney, lung and brain tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 0.1% BSA, 9.85% Tris glycine
PurityTissue culture supernatant
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488) (ab195254)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) (ab195255)
- Anti-ALDH1A1 antibody [EP1933Y] (HRP) (ab195517)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 594) (ab206884)
- Anti-ALDH1A1 antibody [EP1933Y] (Phycoerythrin) (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
Our Abpromise guarantee covers the use of ab52492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
PathwayCofactor metabolism; retinol metabolism.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
- Information by UniProt
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
Tumor tissues of primary invasive ductal carcinomas of the breast were obtained from 192 female patients with stage IIB and III prior to pre-operative neoadjuvant chemotherapy.
(progressive or stable disease, PD/SD)
(partial or complete remission, PR/CR)
The level of ALDH1 was tested by immunohistochemistry staining in paraffin-embedded tissue sections. Rabbit monoclonal ALDH1A1 antibody (ab52492, Abcam) used at a 1:100 dilution.
Sections of the proximal (top) and distal (bottom) mouse colon were assayed for protein expression of ALDH1A1.
Signals yielded by immunohistochemical staining (red) are shown in merged images with DAPI staining (blue). Representative data are shown for three independent experiments. Arrowheads show positive cells for ALDH1A1 in the distal colon. Scale bars, 50 μm.
Colonic tissues were fixed overnight at 4°C in 4% paraformaldehyde, sequentially dehydrated in 10, 15 and 20% sucrose in PBS, and embedded in OCT compound. Colonic organoids were fixed together with surrounding collagen type I gel and processed in the same manner. Frozen sections of 8-μm thickness were subjected to immunohistochemistry using ab52492.
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human kidney tissue sections labeling ALDH1A1 with ab52492.
All lanes : Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Human small intestine tissue lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 1 minute
Blocking/Diluting buffer 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ALDH1A1 with ab52492 at 1/500 dilution (4 μg/ml).
Cells were fixed in 100% methanol. ab150077, an AlexaFluor®488 Goat anti-Rabbit secondary antibody was used at 1/1000 dilution (2 μg/ml). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (2.5 μg/ml). DAPI was used as nuclear counterstain.
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: No staining on MCF-7 cell line.
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human brain tissue sections labeling ALDH1A1 with ab52492.
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human lung tissue sections labeling ALDH1A1 with ab52492.
Immunohistochemical analysis of paraffin-embedded human liver tissue sections labeling ALDH1A1 with ab52492 at 1/100 dilution.
Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab52492 (red line).
The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This product has been referenced in:
- Boulding T et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). Read more (PubMed: 29311580) »
- Shi Y et al. Nuclear EGFR-PKM2 axis induces cancer stem cell-like characteristics in irradiation-resistant cells. Cancer Lett 422:81-93 (2018). WB, ChIP . Read more (PubMed: 29477380) »