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    aldh1a1-antibody-ep1933y-c-terminal-ab52492.pdf

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RecombinantRabMAb

Recombinant Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

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Reviews (22)Q&A (6)References (148)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
  • Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1933Y] to ALDH1A1 - C-terminal
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

You may also be interested in

Protein
Recombinant Human ALDH1A1 protein (ab173068)
Primary
Product image
Anti-CD24 antibody [EPR19925] (ab202073)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ALDH1A1 antibody [EP1933Y] - C-terminal
    See all ALDH1A1 primary antibodies
  • Description

    Rabbit monoclonal [EP1933Y] to ALDH1A1 - C-terminal
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
    Database link: P00352

  • Positive control

    • WB: HepG2 and NIH/3T3 cell lysate; Human small intestine and mouse liver tissue lysate. IHC: Human liver, kidney, lung and brain tissue, human breast carcinoma. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 cell lysate
  • General notes

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1933Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
    • Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y] (ab195255)
    • HRP Anti-ALDH1A1 antibody [EP1933Y] (ab195517)
    • Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y] (ab206884)
    • PE Anti-ALDH1A1 antibody [EP1933Y] (ab209437)
    • Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunohistochemistry kits

    • BrdU Immunohistochemistry Kit (ab125306)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human ALDH1A1 protein (ab173068)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

For unpurified use at 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (3)
1/100 - 1/250.
WB (11)
1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP
1/20 - 1/30.
IHC-P (5)
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Notes
Flow Cyt (Intra)
1/20.

For unpurified use at 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/250.
WB
1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP
1/20 - 1/30.
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Target

  • Function

    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
  • Pathway

    Cofactor metabolism; retinol metabolism.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P00352 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 216 Human
    • Entrez Gene: 11668 Mouse
    • Omim: 100640 Human
    • SwissProt: P00352 Human
    • SwissProt: P24549 Mouse
    • Unigene: 76392 Human
    • Unigene: 250866 Mouse
    • Alternative names

      • Acetaldehyde dehydrogenase 1 antibody
      • AHD2 antibody
      • AL1A1_HUMAN antibody
      • ALDC antibody
      • Aldehyde dehydrogenase 1 family member A1 antibody
      • Aldehyde dehydrogenase 1 soluble antibody
      • Aldehyde dehydrogenase 1A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase cytosolic antibody
      • Aldehyde dehydrogenase family 1 member A1 antibody
      • Aldehyde dehydrogenase liver cytosolic antibody
      • ALDH 1 antibody
      • ALDH 1A1 antibody
      • ALDH class 1 antibody
      • ALDH, liver cytosolic antibody
      • ALDH-E1 antibody
      • ALDH1 A1 antibody
      • ALDH1 antibody
      • ALDH11 antibody
      • ALDH1A1 antibody
      • ALHDII antibody
      • cytosolic antibody
      • epididymis luminal protein 12 antibody
      • epididymis luminal protein 9 antibody
      • epididymis secretory sperm binding protein Li 53e antibody
      • HEL-S-53e antibody
      • MGC2318 antibody
      • PUMB1 antibody
      • RALDH 1 antibody
      • RalDH1 antibody
      • Retinal dehydrogenase 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ALDH1A1 with purified ab52492 at 1/50 dilution (3.54 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      All lanes : Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
      Lane 2 : Human small intestine lysates
      Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg
      Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
      Lane 5 : Mouse liver lysates at 20 µg
      Lane 6 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human kidney tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    • Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      All lanes : Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 2 : Human small intestine tissue lysate
      Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 5 : Mouse liver tissue lysate
      Lane 6 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa


      Exposure time: 1 minute


      Blocking/Diluting buffer 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human brain tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    • Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ALDH1A1 with purified ab52492 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded normal human lung tissue sections labeling ALDH1A1 with ab52492 (unpurified).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)Gong et al PLoS One. 2010 Dec 20;5(12):e15630. doi: 10.1371/journal.pone.0015630. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Tumor tissues of primary invasive ductal carcinomas of the breast were obtained from 192 female patients with stage IIB and III prior to pre-operative neoadjuvant chemotherapy.

      (progressive or stable disease, PD/SD)

      (partial or complete remission, PR/CR)

      The level of ALDH1 was tested by immunohistochemistry staining in paraffin-embedded tissue sections. Rabbit monoclonal ALDH1A1 antibody (unpurified ab52492, Abcam) used at a 1:100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunohistochemical analysis of paraffin-embedded human liver tissue sections labeling ALDH1A1 with ab52492 (unpurified) at 1/100 dilution.

    • Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunoprecipitation - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      ab52492 (purified) at 1/20 dilution (2µg) immunoprecipitating ALDH1A1 in HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates.
      Lane 1: HepG2 whole cell lysates 10µg.
      Lane 2: ab52492 IP in HepG2 whole cell lysates.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52492 in HepG2 whole cell lysates.
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Overlay histogram showingHepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab52492 (unpurified) (red line). 

      The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52492, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ALDH1A1 with ab52492 (purified) at 1/500 dilution (4 μg/ml).

      Cells were fixed in 100% methanol. ab150077, an AlexaFluor®488 Goat anti-Rabbit secondary antibody was used at 1/1000 dilution (2 μg/ml). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (2.5 μg/ml). DAPI was used as nuclear counterstain.

      Confocal image showing cytoplasmic staining on HepG2 cell line.

      Negative control: No staining on MCF-7 cell line.

    • Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
      Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (148)

    Publishing research using ab52492? Please let us know so that we can cite the reference in this datasheet.

    ab52492 has been referenced in 148 publications.

    • Jakob M  et al. Role of cancer stem cell markers ALDH1, BCL11B, BMI-1, and CD44 in the prognosis of advanced HNSCC. Strahlenther Onkol 197:231-245 (2021). PubMed: 32588101
    • Sántha P  et al. Morphological Heterogeneity in Pancreatic Cancer Reflects Structural and Functional Divergence. Cancers (Basel) 13:N/A (2021). PubMed: 33672734
    • Yang N  et al. Cardiac retinoic acid levels decline in heart failure. JCI Insight 6:N/A (2021). PubMed: 33724958
    • Wang Y  et al. Carboxypeptidase A4 negatively correlates with p53 expression and regulates the stemness of breast cancer cells. Int J Med Sci 18:1753-1759 (2021). PubMed: 33746592
    • Pan X  et al. LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis. Cancer Sci 112:2260-2271 (2021). PubMed: 33837611
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-10 of 22 Abreviews

    Western blot abreview for Anti-ALDH1A1 antibody [EP1933Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12% acrylamide gel)
    Sample
    Human Tissue lysate - whole (Brain)
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 24 2013

    Western blot abreview for Anti-ALDH1A1 antibody [EP1933Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Loading amount
    30 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 23 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1A1 antibody [EP1933Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Marta Canamero

    Verified customer

    Submitted Aug 21 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 09 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 25 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Apr 05 2022

    Western blot abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 10 2021

    Western blot abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (22Rv1 prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    22Rv1 prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 10 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human prostate tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Human prostate tissue
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Dec 03 2021

    Flow Cytometry abreview for Anti-ALDH1A1 antibody [EP1933Y] - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (single cells of xenograft human breast cancer cell)
    Permeabilization
    No
    Gating Strategy
    0.5-1%
    Specification
    single cells of xenograft human breast cancer cell
    Preparation
    Cell harvesting/tissue preparation method: Dissociated cells from FFFE xenograft tissues
    Sample buffer: 1% BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    1-10 of 22 Abreviews

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