Recombinant HRP Anti-ALDH1A1 antibody [EP1933Y] (ab195517)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1933Y] to ALDH1A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-ALDH1A1 antibody [EP1933Y]
See all ALDH1A1 primary antibodies -
Description
HRP Rabbit monoclonal [EP1933Y] to ALDH1A1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 whole cell lysate. IHC-P: normal human liver tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1933Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (ab195254)
- Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y] (ab195255)
- Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y] (ab206884)
- PE Anti-ALDH1A1 antibody [EP1933Y] (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
- Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
Notes |
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WB
1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
Target
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- Unigene: 76392 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
see all
Images
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HRP Anti-ALDH1A1 antibody [EP1933Y] (ab195517) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195517 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of ALDH1A1 staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195517 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab195517 has not yet been referenced specifically in any publications.