Product nameAnti-ALDH1A1 antibody [EP1933Y] (HRP)
See all ALDH1A1 primary antibodies
DescriptionRabbit monoclonal [EP1933Y] to ALDH1A1 (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human ALDH1A1 aa 300-400 (C terminal). The exact sequence is proprietary.
- WB: HepG2 whole cell lysate. IHC-P: normal human liver tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 488) (ab195254)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 647) (ab195255)
- Anti-ALDH1A1 antibody [EP1933Y] (Alexa Fluor® 594) (ab206884)
- Anti-ALDH1A1 antibody [EP1933Y] (Phycoerythrin) (ab209437)
- Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free (ab215996)
- Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (ab52492)
Our Abpromise guarantee covers the use of ab195517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
FunctionBinds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
PathwayCofactor metabolism; retinol metabolism.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
- Information by UniProt
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
- AL1A1_HUMAN antibody
Anti-ALDH1A1 antibody [EP1933Y] (HRP) (ab195517) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195517 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of ALDH1A1 staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195517 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab195517 has not yet been referenced specifically in any publications.