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Full length mouse ALDH1A1 protein (GenBank NM_013467) expressed as a fusion protein (Glutathione-S-Transferase) in E. coli.
Our Abpromise guarantee covers the use of ab24343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ALDH1A1 antibody (ab24343) staining [A] tyrosine hydroxylase (TH; green) and [B] ALDH1A1 (red) -containing neurons in adult mouse brain sections by immunohistochemistry. [C] Overlap of TH and ALDH1A1-containing neurons. Pictures were taken with 20x objective. Sections were fixed in 4% formaldehyde for 30 min, rinsed with PBS and then incubated with blocking buffer (PBS, 10 % normal donkey serum; NDS) for 10 min. Sections were then incubated overnight at 4 degrees C with primary antibodies diluted in PBS containing 2% NDS. After additional rinsing in PBS, the coverslips were incubated in fluorescent-labeled secondary antibodies (Cy2-, Rhodamine Red-X-, or Cy5-labeled donkey IgG) in PBS with 2% NDS for 30 min at RT.
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