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Anti-ALDH1A2 antibody (ab96060)

  • Datasheet
  • SDS
Reviews (9)Q&A (1)References (5)

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Western blot - Anti-ALDH1A2 antibody (ab96060)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab96060)

Key features and details

  • Rabbit polyclonal to ALDH1A2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human ALDH1A2 protein (ab125987)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ALDH1A2 antibody
    See all ALDH1A2 primary antibodies
  • Description

    Rabbit polyclonal to ALDH1A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 240-457 of Human ALDH1A2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Visual system
    • Developmental Biology
    • Organogenesis
    • Nervous system development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ALDH1A2 protein (ab125987)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P (1)
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Function

    Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.
  • Pathway

    Cofactor metabolism; retinol metabolism.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O94788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8854 Human
    • Entrez Gene: 19378 Mouse
    • Entrez Gene: 116676 Rat
    • Omim: 603687 Human
    • SwissProt: O94788 Human
    • SwissProt: Q62148 Mouse
    • SwissProt: Q63639 Rat
    • Unigene: 643455 Human
    • Unigene: 42016 Mouse
    • Unigene: 10514 Rat
    see all
  • Alternative names

    • AL1A2_HUMAN antibody
    • Aldehyde dehydrogenase family 1 member A2 antibody
    • ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
    • ALDH1A2 antibody
    • Aldh1a7 antibody
    • AV116159 antibody
    • MGC26444 antibody
    • RALDH 2 antibody
    • RALDH(II) antibody
    • Raldh1 antibody
    • RalDH2 antibody
    • RALDH2 T antibody
    • Retinal dehydrogenase 2 antibody
    • Retinaldehyde dehydrogenase 2 antibody
    • Retinaldehyde specific dehydrogenase type 2 antibody
    • Retinaldehyde-specific dehydrogenase type 2 antibody
    see all

Images

  • Western blot - Anti-ALDH1A2 antibody (ab96060)
    Western blot - Anti-ALDH1A2 antibody (ab96060)
    Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab96060)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab96060)

    Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse testis tissue labeling ALDH1A2 with ab96060 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab96060? Please let us know so that we can cite the reference in this datasheet.

ab96060 has been referenced in 5 publications.

  • Wojahn I  et al. TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung. Respir Res 20:292 (2019). PubMed: 31870435
  • Magella B  et al. Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol 438:84-93 (2018). IHC-P, IF . PubMed: 29596840
  • Hägglund AC  et al. A novel mouse model of anterior segment dysgenesis (ASD): conditional deletion of Tsc1 disrupts ciliary body and iris development. Dis Model Mech 10:245-257 (2017). PubMed: 28250050
  • House CD  et al. NF?B Promotes Ovarian Tumorigenesis via Classical Pathways That Support Proliferative Cancer Cells and Alternative Pathways That Support ALDH+ Cancer Stem-like Cells. Cancer Res 77:6927-6940 (2017). PubMed: 29074539
  • Penny HL  et al. Restoring Retinoic Acid Attenuates Intestinal Inflammation and Tumorigenesis in APCMin/+ Mice. Cancer Immunol Res 4:917-926 (2016). PubMed: 27638841

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 04 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 04 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 08 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (MDBK)
Specification
MDBK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 08 2012

Western blot abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
100 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 31 2012

Western blot abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK)
Loading amount
100 µg
Specification
MDBK
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 30 2012

Western blot abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 30 2012

Western blot abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3)
Loading amount
100 µg
Specification
3T3
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 30 2012

Western blot abreview for Anti-ALDH1A2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse liver and kidney extract)
Loading amount
100 µg
Specification
Mouse liver and kidney extract
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 28 2012

Question

What was the adjuvant, if any, that was used to generate this antibody?

Read More

Abcam community

Verified customer

Asked on Nov 22 2011

Answer

Following up my previous message, the developer of the Anti-ALDH1A2 antibody, ab96060, tells us that it was generated using a combination of complete and incomplete Freund’s adjuvant. However, the exact formulation is proprietary. I hope this information is of use to you. Please let me know if you have any other questions.

Read More

Abcam Scientific Support

Answered on Nov 22 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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