- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-ALDH1A2 antibody
See all ALDH1A2 primary antibodies -
DescriptionRabbit polyclonal to ALDH1A2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Fr, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 240-457 of Human ALDH1A2.
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Positive control
- IMR32 whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab96060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use a concentration of 5 µg/ml. |
Target
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FunctionRecognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.
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PathwayCofactor metabolism; retinol metabolism.
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Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 8854 Human
- Entrez Gene: 19378 Mouse
- Entrez Gene: 116676 Rat
- Omim: 603687 Human
- SwissProt: O94788 Human
- SwissProt: Q62148 Mouse
- SwissProt: Q63639 Rat
- Unigene: 643455 Human
see all -
Alternative names
- AL1A2_HUMAN antibody
- Aldehyde dehydrogenase family 1 member A2 antibody
- ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
see all
Images
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Immunoprecipitation - Anti-ALDH1A2 antibody (ab96060)ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 57kDa; ALDH1A2
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Western blot - Anti-ALDH1A2 antibody (ab96060)Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 57 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A2 antibody (ab96060)Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse testis tissue labeling ALDH1A2 with ab96060 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Hägglund AC et al. A novel mouse model of anterior segment dysgenesis (ASD): conditional deletion of Tsc1 disrupts ciliary body and iris development. Dis Model Mech 10:245-257 (2017). Read more (PubMed: 28250050) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"