Product nameAnti-ALDH1A2 antibody
See all ALDH1A2 primary antibodies
DescriptionRabbit polyclonal to ALDH1A2
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC-Fr, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment, corresponding to a region within amino acids 240-457 of Human ALDH1A2.
- IMR32 whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
FunctionRecognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.
PathwayCofactor metabolism; retinol metabolism.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
- Information by UniProt
- AL1A2_HUMAN antibody
- Aldehyde dehydrogenase family 1 member A2 antibody
- ALDH1A2 aldehyde dehydrogenase 1 family, member A2 antibody
Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 57 kDa
10% SDS PAGE
ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 57kDa; ALDH1A2
Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse testis tissue labeling ALDH1A2 with ab96060 at 1/500 dilution.
This product has been referenced in:
- Magella B et al. Reduced Abd-B Hox function during kidney development results in lineage infidelity. Dev Biol 438:84-93 (2018). IHC-P, IF . Read more (PubMed: 29596840) »
- Hägglund AC et al. A novel mouse model of anterior segment dysgenesis (ASD): conditional deletion of Tsc1 disrupts ciliary body and iris development. Dis Model Mech 10:245-257 (2017). Read more (PubMed: 28250050) »