Overview

  • Product name

    Anti-ALDH1L1 antibody [2A6]
    See all ALDH1L1 primary antibodies
  • Description

    Mouse monoclonal [2A6] to ALDH1L1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Dog, African green monkey
  • Immunogen

    Recombinant full length protein corresponding to Human ALDH1L1.
    Database link: O75891

  • Positive control

    • WB: HepG2, HeLa, HT-29, A549, COS-7, MDCK, PC-12 and pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate. IHC-P: Human kidney and liver tissue. Flow cyt: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab231018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB 1/500 - 1/2000.
Flow Cyt 1/100.

Target

  • Tissue specificity

    Highly expressed in liver, pancreas and kidney.
  • Sequence similarities

    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for ALDH1L1 using ab231018 at 10 μg/ml in immunohistochemical analysis.

  • All lanes : Anti-ALDH1L1 antibody [2A6] (ab231018) at 1/500 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY ALDH1L1-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.
  • All lanes : Anti-ALDH1L1 antibody [2A6] (ab231018) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK (canine kidney cell line) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.
  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling ALDH1L1 with ab231018 at 1/100 dilution (red) compared with a non-specific negative control antibody (blue).

References

ab231018 has not yet been referenced specifically in any publications.

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