Anti-ALDH1L1 antibody [3E9] (ab56777)
Key features and details
- Mouse monoclonal [3E9] to ALDH1L1
- Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG3
Overview
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Product name
Anti-ALDH1L1 antibody [3E9]
See all ALDH1L1 primary antibodies -
Description
Mouse monoclonal [3E9] to ALDH1L1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit -
Immunogen
Recombinant fragment: EESFGPVMII SRFADGDLDA VLSRANATEF GLASGVFTRD INKALYVSDK LQAGTVFVNT YNKTDVAAPF GGFKQSGFGK DLGEAALNEY LRVKTVTFEY , corresponding to amino acids 803-903 of Human ALDH1L1
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General notes
This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
3E9 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 99 kDa.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 99 kDa. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Highly expressed in liver, pancreas and kidney. -
Sequence similarities
In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Contains 1 acyl carrier domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10840 Human
- Entrez Gene: 107747 Mouse
- Omim: 600249 Human
- SwissProt: O75891 Human
- SwissProt: Q8R0Y6 Mouse
- Unigene: 434435 Human
- Unigene: 30035 Mouse
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Alternative names
- 10 formyltetrahydrofolate dehydrogenase antibody
- 10 FTHFDH antibody
- 10-formyltetrahydrofolate dehydrogenase antibody
see all
Images
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ALDH1L1 antibody (ab56777) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
This image was generated using the ascites version of the product.
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ALDH1L1 antibody (ab56777) at 1ug/lane + NIH/3T3 cell lysate at 25 μg/lane.
This image was generated using the ascites version of the product.
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ICC/IF image of ab56777 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Overlay histogram showing HepG2 cells stained with ab56777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56777, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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ALDH1L1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to ALDH1L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56777.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 99kDa: ALDH1L1; non specific - 60kDa: We are unsure as to the identity of this extra band.This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (24)
ab56777 has been referenced in 24 publications.
- Chang KC et al. The chromatin repressors EZH2 and Suv4-20h coregulate cell fate specification during hippocampal development. FEBS Lett 596:294-308 (2022). PubMed: 34890048
- Wang W et al. The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse. EMBO Rep 23:e53602 (2022). PubMed: 34935271
- Cheng YC et al. Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation. Stem Cell Rev Rep 18:839-852 (2022). PubMed: 35061207
- Wang W et al. Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development. Adv Sci (Weinh) 9:e2105208 (2022). PubMed: 35488517
- Cornelison RC et al. A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment. NPJ Precis Oncol 6:54 (2022). PubMed: 35906273