Overview

  • Product name

  • Description

    Goat polyclonal to ALDH1L1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human, Orangutan
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Recombinant mouse ALDH1L1 protein; Mouse liver tissue lysate. ICC/IF: NIH/3T3 cells. IHC-P: Mouse kidney and liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 99 kDa.
ICC/IF Use a concentration of 15 µg/ml.
IHC-P 1/200.

HIER using Citrate Buffer for 20 minutes.

Target

  • Tissue specificity

    Highly expressed in liver, pancreas and kidney.
  • Sequence similarities

    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

Images

  • All lanes : Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution

    Lane 1 : Opal pre-stained ladder
    Lane 2 : Recombinant mouse ALDH1L1 protein
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 5 : Mouse liver (normal tissue) lysate

    Secondary
    Lanes 1-4 : F(ab)'2 Dk-a-Gt IgG [H&L] at 1/40000 dilution
    Lane 5 : Goat polyclonal to ALDH1L1 (ab235197) at 1/40000 dilution (F(ab)'2 Dk-a-Gt IgG [H&L])

    Predicted band size: 99 kDa
    Observed band size: 98.7 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).

    Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

    A) DAPI
    B) ab235197 + secondary.
    C) Merged image
    D) Secondary only control.

    Expected localization: Cytoplasm.

  • 100% methanol-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ALDH1L1 (Green) using ab235197 at 15 μg/mL over night at 4°C in ICC/IF. The nuclear counterstain is DAPI (Blue).

    Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

    A) DAPI
    B) ab235197 + secondary.
    C) Merged image
    D) Secondary only control.

    Expected localization: Cytoplasm.

  • Immunohistochemical analysis of mouse kidney tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.

    Pathologist analysis: ALDH1L1 showed selective staining of renal tubular epithelial cells in mouse kidney. The specificity of the staining is cytoplasmic and membranous in tubules not in glomeruli which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1:200. 

  • Immunohistochemical analysis of mouse liver tissue labeling ALDH1L1 with ab235197 at 1/200 dilution, followed by a Donkey Anti-Goat IgG (HRP) secondary antibody for 20 minutes at room temperature. Images are shown at 20x (lower left) and 40x (lower right) magnification. Counter stained with Hematoxylin. Upper left panel, H&E staining.

    Pathologist analysis: ALDH1L1 showed selective cytoplasmic staining in mouse liver of hepatocytes. The specificity of the staining is weak in bile duct cells and strong membranous and cytoplasmic in hepatocytes which is consistent with ALDH1L1 staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1:200. 

  • All lanes : Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : ALDH1L1 HEK293T overexpressed lysate

    Secondary
    All lanes : 605-703-125 GOAT IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed at 1/40000 dilution

    Predicted band size: 99 kDa


    Exposure time: 1 second


    ALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).

  • Anti-ALDH1L1 antibody (ab235197) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Secondary
    Goat polyclonal to ALDH1L1 (ab235197) at 1/40000 dilution (605-703-125 GOAT IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed)

    Predicted band size: 99 kDa
    Observed band size: 80, 34 kDa why is the actual band size different from the predicted?


    Exposure time: 35 seconds


    ALDH1L1 detection in NIH-3T3 by WB weak under general lysate conditions. Optimal detection of protein in 3T3 cells at G2/M enriched cells (not tested).

References

ab235197 has not yet been referenced specifically in any publications.

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