Overview

  • Product name
  • Description
    Mouse monoclonal to ALDH1L1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant fragment: EESFGPVMII SRFADGDLDA VLSRANATEF GLASGVFTRD INKALYVSDK LQAGTVFVNT YNKTDVAAPF GGFKQSGFGK DLGEAALNEY LRVKTVTFEY , corresponding to amino acids 803-903 of Human ALDH1L1

Properties

Applications

Our Abpromise guarantee covers the use of ab56777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

Target

  • Tissue specificity
    Highly expressed in liver, pancreas and kidney.
  • Sequence similarities
    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

Images

  • ALDH1L1 antibody (ab56777) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
  • ALDH1L1 antibody (ab56777) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab56777 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab56777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56777, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ALDH1L1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to ALDH1L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56777.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 99kDa: ALDH1L1; non specific - 60kDa: We are unsure as to the identity of this extra band.

References

This product has been referenced in:
  • Garcia-Esparcia P  et al. Glutamate Transporter GLT1 Expression in Alzheimer Disease and Dementia With Lewy Bodies. Front Aging Neurosci 10:122 (2018). Read more (PubMed: 29755340) »
  • Chen J  et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3:17044 (2017). Read more (PubMed: 29238610) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Syrian Hamster Cell (Mixed glial culture)
Specification
Mixed glial culture
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 15 2013

Answer

Considering that you would like to perform IHC on rabbit specimen, I agree that it is advisable to avoid antibodies generated in rabbit. Therefore, although the MMP12 antibody ab56305 shows a rather low sequence similarity across the full-length protein, it might indeed be the most appropriate of our MMP12 antibodies to test, as it is a mouse monoclonal antibody that is guaranteed to work in IHC-P. We have also a chicken polyclonal and a goat polyclonal anti-MMP12 antibody available. As these are not yet tested in IHC though, they might be a better option to test later, in case ab56305 might not work. DISCOUNT CODES: ab70191:xxx ab56777:xxx ab6328: xxx ab56305: xxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to accept our offer and test these antibodies in rabbit. This code will give you 1 free primary antibody each before the expiration date. To redeem this offer, please submit an Abreview for rabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our antibodies. The immunogen and epitope sequences, respectively, show the following similarities against rabbit: 1) ab70191: 93% 2) ab56777: 96% 3) ab56305: 72% 4) ab6328: 100% Due to this high homology, the anti-Lumican antibody, the anti-ALDH1L1 antibody and the anti-Fibronectin antibody are likely to cross-react with the rabbit orthologs, whereas the homology for the MMP12 antibody seems to low which is why we would not recommend testing this antibody. However, if requested I would be pleased to look for another anti-MMP12 antibody that might be suitable for rabbit, as we have for example antibody against the N- or C-terminus of the protein. To our knowledge, ab70191, ab56777 and ab6328 have not been tested in rabbit yet. Therefore, I can offer a discount off a future purchase if you buy ab70191, ab56777 and/or ab6328 now, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody each. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab70191, ab56777 and/or ab6328 in rabbit. I will then send a discount code. This code must be issued before purchasing ab70191, ab56777 and/or ab6328 so please wait for my reply before ordering. 2. Purchase ab either by phone, fax, or online (www.abcam.com). 3. Test it in rabbit. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab70191, ab56777 and/or ab6328 turn out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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