Overview

  • Product name
    Anti-ALDH1L1 antibody - Astrocyte Marker
    See all ALDH1L1 primary antibodies
  • Description
    Rabbit polyclonal to ALDH1L1 - Astrocyte Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Mouse ALDH1L1 aa 300-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q8R0Y6
    (Peptide available as ab103935)

  • Positive control
    • WB: Mouse liver; mouse brain and rat brain lysates. IHC-P: Mouse brain tissue. IHC-Fr: Mouse brain-astrocytes tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab87117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml. PubMed: 24501362
IHC-Fr 1/1000.
ICC Use at an assay dependent concentration.

Target

  • Tissue specificity
    Highly expressed in liver, pancreas and kidney.
  • Sequence similarities
    In the N-terminal section; belongs to the GART family.
    In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
    Contains 1 acyl carrier domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10 formyltetrahydrofolate dehydrogenase antibody
    • 10 FTHFDH antibody
    • 10-formyltetrahydrofolate dehydrogenase antibody
    • 10-FTHFDH antibody
    • AL1L1_HUMAN antibody
    • Aldehyde dehydrogenase 1 family member L1 antibody
    • Aldehyde dehydrogenase family 1 member L1 antibody
    • aldh1l1 antibody
    • Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
    • DKFZp781N0997 antibody
    • EC 1.5.1.6 antibody
    • FDH antibody
    • formyltetrahydrofolate dehydrogenase antibody
    • FTHFD antibody
    • OTTHUMP00000155221 antibody
    • OTTHUMP00000214696 antibody
    • OTTHUMP00000214700 antibody
    • OTTHUMP00000222724 antibody
    see all

Images

  • PF-PBS post-fixed lamina frozen sections treated with rNAION for 7 days to induce ischaemia, were analyzed for ALDH1L1 (Red)(ab87117) at 1:1000 dilution. Antigen recovery using citrate buffer was performed. There is extensive dendrimer signal overlap with the astrocyte cytoplasm (arrows).

  • All lanes : Anti-ALDH1L1 antibody - Astrocyte Marker (ab87117) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • IHC-P image of ALDH1L1 staining on Mouse brain tissue section using ab87117. The sections were subjected to heat-mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 minutes at 21°C and then incubated with ab87117 (1/1000 dilution) for 2 hours at 21°C.

    See Abreview

  • ab87117 staining ALDH1L1 in mouse brain-astrocytes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, incubated with 30% sucrose, embedded in OCT and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.3% Triton + 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Paraffin-embedded rat brain tissue stained for ALDH1L1 using ab87117 at 1/1000 dilution in immunohistochemical analysis.

    Astrocytes are clearly labeled: red for those attaching to the pia mater, blue for those in the grey matter and green for astrocytes that are intimately associated with blood vessels.

    See Abreview

References

This product has been referenced in:
See all 19 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 08 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Mouse Tissue sections (brain-astrocytes)
Specification
brain-astrocytes
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 05 2014

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (mouse cortex)
Specification
mouse cortex
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton x100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 29 2012

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Astrocytes)
Permeabilization
Yes - Triton-X100
Specification
Astrocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde

Rehana Leak

Verified customer

Submitted Jul 01 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain cortex)
Specification
Brain cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted May 03 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Feb 24 2011

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