Key features and details
- Mouse monoclonal [9A10AF10] to ALDH2
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Product nameAnti-ALDH2 antibody [9A10AF10]
See all ALDH2 primary antibodies
DescriptionMouse monoclonal [9A10AF10] to ALDH2
Tested applicationsSuitable for: ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Tissue, cells or virus. This information is considered to be commercially sensitive.
- HepG2 cells; Human, Rat and Mouse liver and HepG2 whole cell lysate (ab7900).
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesab110311 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab110311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml. For 2 hours.|
|IP||Use at an assay dependent concentration.|
PathwayAlcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
Immunocytochemistry analysis using ab110311 at 5µg/ml staining ALDH2 in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: Target protein locates mainly in the mitochondrial matrix.
Using MitoSciences' standard immunoprecipitation protocol, ab110311 precipitates down the 54kDa mature ALDH2 protein from human, mouse, and rat liver tissue lysate and HepG2 culture cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
ab110311 has not yet been referenced specifically in any publications.