Product nameAnti-ALDH2 antibody
See all ALDH2 primary antibodies
DescriptionRabbit polyclonal to ALDH2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human ALDH2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P05091
- This antibody gave a positive signal in the following tissue lysates: Human Liver; Mouse Liver; Rat Liver; Human Skin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab154993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
PathwayAlcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
All lanes : Anti-ALDH2 antibody (ab154993) at 1/250 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : Human skin tissue lysate - total protein (ab30166)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 70 kDa (possible non-specific binding)
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154993 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab154993 has not yet been referenced specifically in any publications.