Anti-ALDH2 antibody (ab227021)
Key features and details
- Rabbit polyclonal to ALDH2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ALDH2 antibody
See all ALDH2 primary antibodies -
Description
Rabbit polyclonal to ALDH2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment within Human ALDH2 (internal sequence). The exact sequence is proprietary.
Database link: P05091 -
Positive control
- ICC/IF: A549 cells. IHC-P: Mouse prostate and colon tissues; AGS xenograft tissue. WB: HepG2 whole cell and cytoplasmic extracts; GL261, C8D30, BCL-1 and RAW 264.7 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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ICC/IF | (2) |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Pathway
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 508629 Cow
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 733685 Pig
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P20000 Cow
- SwissProt: P05091 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
Images
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All lanes : Anti-ALDH2 antibody (ab227021) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa10% SDS-PAGE gel.
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A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.
Blue: Hoechst 33342 staining.
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Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-ALDH2 antibody (ab227021) at 1/1000 dilution
Lane 1 : GL261 whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227021 has been referenced in 1 publication.
- Jin N et al. Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway. J Cell Biochem 119:6882-6893 (2018). PubMed: 29665144