Overview

  • Product name

  • Description

    Rabbit polyclonal to ALDH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human ALDH2 (internal sequence). The exact sequence is proprietary.
    Database link: P05091

  • Positive control

    • ICC/IF: A549 cells. IHC-P: Mouse prostate and colon tissues; AGS xenograft tissue. WB: HepG2 whole cell and cytoplasmic extracts; GL261, C8D30, BCL-1 and RAW 264.7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab227021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-ALDH2 antibody (ab227021) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa



    10% SDS-PAGE gel.

  • A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-ALDH2 antibody (ab227021) at 1/1000 dilution

    Lane 1 : GL261 whole cell lysate
    Lane 2 : C8D30 whole cell lysate
    Lane 3 : BCL-1 whole cell lysate
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.

References

ab227021 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227021.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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