Overview

  • Product name

    Anti-ALDH2 antibody [EPR4493]
    See all ALDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4493] to ALDH2
  • Host species

    Rabbit
  • Specificity

    The antibody immunogen has 82% homology with ALDH1B1 (P30837) and 77% with ALDH1A1 (P00352). BLAST results show for both proteins 6 aa in a row with 100% homology, therefore a cross-reactivity with these proteins is likely.
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, Mouse liver, and Rat liver lysates. IHC-P: human lung carcinoma, mouse colon, and rat stomach tissues. IP: Mouse liver cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab108306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/2400.

Antigen retrieval is recommended.

For unpurified use at 1/50 - 1/100.

See IHC antigen retrieval protocols.

Target

  • Pathway

    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

Images

  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/2000 dilution (Purified)

    Lane 1 : Mouse liver lysates
    Lane 2 : Rat liver lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling ALDH2 with purified ab108306 at 1/2400 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution ((unpurified))

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • ab108306 (purified ) at 1/20 dilution (1ug) immunoprecipitating ALDH2 in Mouse liver lysate.
    Lane 1 (input): Mouse liver lysate 10ug
    Lane 2 (+): ab108306 & Mouse liver lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108306 in Mouse liver lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP)�(ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution ((unpurified))

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling ALDH2 with purified ab108306 at 1/2400 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ALDH2 with purified ab108306 at 1/2400 dilution (0.07 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Unpurified ab108306 at 1/50 dilution, staining ALDH2 in paraffin-embedded Human colon tissue

References

This product has been referenced in:

  • Hao L  et al. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease. Redox Biol 14:626-636 (2018). WB, IP ; Mouse . Read more (PubMed: 29156373) »
  • Liu W  et al. Aldehyde dehydrogenase 2 activation ameliorates cyclophosphamide-induced acute cardiotoxicity via detoxification of toxic aldehydes and suppression of cardiac cell death. J Mol Cell Cardiol 121:134-144 (2018). Read more (PubMed: 29981795) »
See all 13 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for your enquiry and your interest in our products. Here are the answers to your questions: 1) As the datasheet indicates this antibody is tissue culture supernatant and not affinity purified. Therefore the IgG concentration has not been quantified. For IP, we recommend diluting it at 1/10 - 1/100. 2) As general guidance, we can only provide the approximate range of IgG concentration (1 - 3 mg/ml) but not the exact value. If you need any further assistance in the future, please do not hesitate to contact me.

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