Overview

  • Product name
    Anti-ALDH2 antibody [EPR4493]
    See all ALDH2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4493] to ALDH2
  • Host species
    Rabbit
  • Specificity
    The antibody immunogen has 82% homology with ALDH1B1 (P30837) and 77% with ALDH1A1 (P00352). BLAST results show for both proteins 6 aa in a row with 100% homology, therefore a cross-reactivity with these proteins is likely.
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: HepG2 and A549 cell lysates and mouse liver and rat liver tissue lysates. IHC-P: Human colon tissue. Flow Cyt: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab108306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC 1/100 - 1/250.

Target

  • Pathway
    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities
    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

Images

  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • ab108306 at 1/50 dilution, staining ALDH2 in paraffin-embedded Human colon tissue
  • Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ALDH2 (red) with ab108306 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • All lanes : Anti-ALDH2 antibody [EPR4493] (ab108306) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

References

This product has been referenced in:
  • Hao L  et al. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease. Redox Biol 14:626-636 (2018). WB, IP ; Mouse . Read more (PubMed: 29156373) »
  • Liu W  et al. Aldehyde dehydrogenase 2 activation ameliorates cyclophosphamide-induced acute cardiotoxicity via detoxification of toxic aldehydes and suppression of cardiac cell death. J Mol Cell Cardiol 121:134-144 (2018). Read more (PubMed: 29981795) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Question
Answer

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for your enquiry and your interest in our products. Here are the answers to your questions: 1) As the datasheet indicates this antibody is tissue culture supernatant and not affinity purified. Therefore the IgG concentration has not been quantified. For IP, we recommend diluting it at 1/10 - 1/100. 2) As general guidance, we can only provide the approximate range of IgG concentration (1 - 3 mg/ml) but not the exact value. If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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