Recombinant Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-ALDH2 antibody [EPR4493] (HRP)
See all ALDH2 primary antibodies -
Description
Rabbit monoclonal [EPR4493] to ALDH2 (HRP) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.
-
Positive control
- WB: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4493 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab199210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
-
Pathway
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
- SwissProt: P11884 Rat
- Unigene: 604551 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
Images
-
![Western blot - Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)](https://www.abcam.com/ps/products/199/ab199210/Images/ab199210-251067-anti-aldh2-antibody-epr4493-hrp-western-blot.jpg)
Western blot - Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210) at 1/5000 dilution + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199210 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
References
ab199210 has not yet been referenced specifically in any publications.
