Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4493] to ALDH2 (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-ALDH2 antibody [EPR4493] (HRP)
See all ALDH2 primary antibodies
DescriptionRabbit monoclonal [EPR4493] to ALDH2 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.
- WB: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab199210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
PathwayAlcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Sequence similaritiesBelongs to the aldehyde dehydrogenase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210) at 1/5000 dilution + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199210 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab199210 has not yet been referenced specifically in any publications.