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  1. Link

    aldh2-antibody-epr4493-hrp-ab199210.pdf

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Recombinant
RabMAb

Recombinant Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)

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  • Western blot - Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-ALDH2 antibody [EPR4493] (HRP)
    See all ALDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4493] to ALDH2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ALDH2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4493
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-ALDH2 antibody [EPR4493] (ab108306)
    • Anti-ALDH2 antibody [EPR4493] (Biotin) (ab199742)
    • Anti-ALDH2 antibody [EPR4493] - BSA and Azide free (ab239925)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (HRP) (ab199507)
  • Recombinant Protein

    • Recombinant human ALDH2 protein (Active) (ab87415)

Applications

Our Abpromise guarantee covers the use of ab199210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Pathway

    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P05091 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 217 Human
    • Entrez Gene: 11669 Mouse
    • Entrez Gene: 29539 Rat
    • Omim: 100650 Human
    • SwissProt: P05091 Human
    • SwissProt: P47738 Mouse
    • SwissProt: P11884 Rat
    • Unigene: 604551 Human
    • Unigene: 284446 Mouse
    • Unigene: 101781 Rat
    see all
  • Alternative names

    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

Images

  • Western blot - Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)
    Western blot - Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210)
    Anti-ALDH2 antibody [EPR4493] (HRP) (ab199210) at 1/5000 dilution + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199210 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

Protocols

  • Western blot protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab199210 has not yet been referenced specifically in any publications.

    Publishing research using ab199210? Please let us know so that we can cite the reference in this datasheet.

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