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  1. Link

    aldh2-antibody-epr4494-ab133306.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ALDH2 antibody [EPR4494] (ab133306)

  • Datasheet
  • SDS
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Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
  • Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
  • Anti-ALDH2 antibody [EPR4494] (ab133306)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4494] to ALDH2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Knockout
Product image
Human ALDH2 knockout SW480 cell lysate (ab270506)
Assay
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ALDH2 Inhibitor Screening Kit (Fluorometric) (ab283364)
Protein
Product image
Recombinant human ALDH2 protein (Active) (ab87415)

View more associated products

Overview

  • Product name

    Anti-ALDH2 antibody [EPR4494]
    See all ALDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4494] to ALDH2
  • Host species

    Rabbit
  • Specificity

    The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480 and HepG2 cell lysates; Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4494
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-ALDH2 antibody [EPR4494] - BSA and Azide free (ab248459)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ALDH2 knockout SW480 cell line (ab270483)
  • KO cell lysates

    • Human ALDH2 knockout SW480 cell lysate (ab270506)
  • Recombinant Protein

    • Recombinant human ALDH2 protein (Active) (ab87415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Pathway

    Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P05091 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 217 Human
    • Entrez Gene: 11669 Mouse
    • Entrez Gene: 29539 Rat
    • Omim: 100650 Human
    • SwissProt: P05091 Human
    • SwissProt: P47738 Mouse
    • SwissProt: P11884 Rat
    • Unigene: 604551 Human
    • Unigene: 284446 Mouse
    • Unigene: 101781 Rat
    see all
  • Alternative names

    • Acetaldehyde dehydrogenase 2 antibody
    • Aldehyde dehydrogenase 2 family (mitochondrial) antibody
    • Aldehyde dehydrogenase 2 family antibody
    • Aldehyde dehydrogenase mitochondrial antibody
    • Aldehyde dehydrogenase, mitochondrial antibody
    • ALDH 2 antibody
    • ALDH class 2 antibody
    • ALDH E2 antibody
    • ALDH-E2 antibody
    • Aldh2 antibody
    • ALDH2_HUMAN antibody
    • ALDHI antibody
    • ALDM antibody
    • Liver mitochondrial ALDH antibody
    • MGC1806 antibody
    • Mitochondrial aldehyde dehydrogenase 2 antibody
    • MS767 antibody
    • Nucleus encoded mitochondrial aldehyde dehydrogenase 2 antibody
    see all

Images

  • Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

    Lane 1 : Wild-type SW480 cell lysate
    Lane 2 : ALDH2 knockout SW480 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

    Lane 1 : HepG2 lysates
    Lane 2 : Human heart lysates
    Lane 3 : Fetal liver lysates
    Lane 4 : Mouse liver lysates
    Lane 5 : Rat liver lysates
    Lane 6 : Mouse lung lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa

  • Anti-ALDH2 antibody [EPR4494] (ab133306)
    Anti-ALDH2 antibody [EPR4494] (ab133306)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab133306? Please let us know so that we can cite the reference in this datasheet.

ab133306 has been referenced in 1 publication.

  • Bardallo RG  et al. Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach. Int J Mol Sci 22:N/A (2021). PubMed: 34069402

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