Recombinant
Recombinant Anti-ALDH2 antibody [EPR4494] (ab133306)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ALDH2 antibody [EPR4494]
See all ALDH2 primary antibodies -
Description
Rabbit monoclonal [EPR4494] to ALDH2 -
Host species
Rabbit -
Specificity
The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely. -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human ALDH2 (P05091).
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Positive control
- HepG2, Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4494 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 56 kDa. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Pathway
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
- SwissProt: P11884 Rat
- Unigene: 604551 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
Images
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![Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)](https://www.abcam.com/ps/products/133/ab133306/Images/ab133306-146797-anti-aldh2-antibody-epr4494-western-blot.jpg)
Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : HepG2 lysates
Lane 2 : Human heart lysates
Lane 3 : Fetal liver lysates
Lane 4 : Mouse liver lysates
Lane 5 : Rat liver lysates
Lane 6 : Mouse lung lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Datasheets and documents
References
ab133306 has not yet been referenced specifically in any publications.
