Recombinant Anti-ALDH2 antibody [EPR4494] (ab133306)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4494] to ALDH2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ALDH2 antibody [EPR4494]
See all ALDH2 primary antibodies -
Description
Rabbit monoclonal [EPR4494] to ALDH2 -
Host species
Rabbit -
Specificity
The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human ALDH2 (P05091).
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Positive control
- WB: SW480 and HepG2 cell lysates; Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4494 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
Target
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Pathway
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P05091 Human
- SwissProt: P47738 Mouse
- SwissProt: P11884 Rat
- Unigene: 604551 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
Images
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![Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)](https://www.abcam.com/ps/products/133/ab133306/Images/ab133306-396483-anti-aldh2-antibody-epr4494-western-blot-sw480-wt-hepg2-mcf7-sw480-aldh2-knockout.jpg)
Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ALDH2 knockout SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)](https://www.abcam.com/ps/products/133/ab133306/Images/ab133306-146797-anti-aldh2-antibody-epr4494-western-blot.jpg)
Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution
Lane 1 : HepG2 lysates
Lane 2 : Human heart lysates
Lane 3 : Fetal liver lysates
Lane 4 : Mouse liver lysates
Lane 5 : Rat liver lysates
Lane 6 : Mouse lung lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa -
![Anti-ALDH2 antibody [EPR4494] (ab133306)](https://www.abcam.com/ps/products/133/ab133306/Images/ab133306-3-benefits-of-recombinant-antibodies.png)
Anti-ALDH2 antibody [EPR4494] (ab133306)
Datasheets and documents
References (0)
ab133306 has not yet been referenced specifically in any publications.
