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Signal Transduction Metabolism Energy Metabolism
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Anti-ALDH3A1 antibody (ab75388)

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Western blot - Anti-ALDH3A1 antibody (ab75388)

    Key features and details

    • Rabbit polyclonal to ALDH3A1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human ALDH3A1 protein (ab93463)

    View more associated products

    Overview

    • Product name

      Anti-ALDH3A1 antibody
      See all ALDH3A1 primary antibodies
    • Description

      Rabbit polyclonal to ALDH3A1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Dog
    • Immunogen

      Synthetic peptide corresponding to Human ALDH3A1 aa 1-100 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab90603)

    • Positive control

      • This antibody gave a positive signal in human stomach tumour tissue lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Neuroscience
      • Sensory System
      • Visual system
      • Signal Transduction
      • Metabolism
      • Alcohol metabolism
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Alcohol metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant human ALDH3A1 protein (ab93463)
    • Recombinant Protein

      • Recombinant human ALDH3A1 protein (ab93463)

    Applications

    Our Abpromise guarantee covers the use of ab75388 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Target

    • Function

      ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
    • Tissue specificity

      High levels in stomach, esophagus and lung; low level in the liver and kidney.
    • Sequence similarities

      Belongs to the aldehyde dehydrogenase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P30838 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 489526 Dog
      • Entrez Gene: 218 Human
      • Omim: 100660 Human
      • SwissProt: P30838 Human
      • Unigene: 531682 Human
      • Alternative names

        • Acetaldehyde dehydrogenase 3 antibody
        • AHD 4 antibody
        • AHD C antibody
        • AHD4 antibody
        • AHDC antibody
        • AL3A1_HUMAN antibody
        • Aldehyde dehydrogenase 3 antibody
        • Aldehyde dehydrogenase 3 family member A1 antibody
        • Aldehyde dehydrogenase 3A1 antibody
        • Aldehyde dehydrogenase antibody
        • Aldehyde dehydrogenase class 3 antibody
        • Aldehyde dehydrogenase dimeric NADP preferring antibody
        • Aldehyde dehydrogenase family 3 member A1 antibody
        • Aldehyde dehydrogenase family 3 subfamily A1 antibody
        • Aldehyde dehydrogenase family 3, subfamily A, member 1 antibody
        • Aldehyde dehydrogenase isozyme 3 antibody
        • Aldehyde dehydrogenase type III antibody
        • ALDH 3 antibody
        • ALDH 3A1 antibody
        • ALDH III antibody
        • ALDH, stomach type antibody
        • ALDH3 A1 antibody
        • ALDH3 antibody
        • ALDH3A 1 antibody
        • Aldh3a1 antibody
        • ALDHIII antibody
        • dimeric NADP-preferring antibody
        • MGC10406 antibody
        • Stomach aldehyde dehydrogenase antibody
        • Tumor associated aldehyde dehydrogenase tumor ALDH antibody
        see all

      Images

      • Western blot - Anti-ALDH3A1 antibody (ab75388)
        Western blot - Anti-ALDH3A1 antibody (ab75388)
        Anti-ALDH3A1 antibody (ab75388) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 50 kDa
        Observed band size: 51 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 8 minutes

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab75388? Please let us know so that we can cite the reference in this datasheet.

      ab75388 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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