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RecombinantRabMAb

Recombinant Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

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Western blot - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
  • Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ALDH3A1 antibody [SP298] - C-terminal
    See all ALDH3A1 primary antibodies

  • Description

    Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 cell lysate. IHC-P: Human bladder, esophagus, skin, cervix, tonsil, stomach, stomach adenocarcinoma, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/400. Predicted molecular weight: 50 kDa.
IHC-P
1/100.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.
Notes
WB
1/400. Predicted molecular weight: 50 kDa.
IHC-P
1/100.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.

Target

  • Function

    ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

  • Tissue specificity

    High levels in stomach, esophagus and lung; low level in the liver and kidney.

  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Acetaldehyde dehydrogenase 3 antibody
      • AHD 4 antibody
      • AHD C antibody
      • AHD4 antibody
      • AHDC antibody
      • AL3A1_HUMAN antibody
      • Aldehyde dehydrogenase 3 antibody
      • Aldehyde dehydrogenase 3 family member A1 antibody
      • Aldehyde dehydrogenase 3A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase class 3 antibody
      • Aldehyde dehydrogenase dimeric NADP preferring antibody
      • Aldehyde dehydrogenase family 3 member A1 antibody
      • Aldehyde dehydrogenase family 3 subfamily A1 antibody
      • Aldehyde dehydrogenase family 3, subfamily A, member 1 antibody
      • Aldehyde dehydrogenase isozyme 3 antibody
      • Aldehyde dehydrogenase type III antibody
      • ALDH 3 antibody
      • ALDH 3A1 antibody
      • ALDH III antibody
      • ALDH, stomach type antibody
      • ALDH3 A1 antibody
      • ALDH3 antibody
      • ALDH3A 1 antibody
      • Aldh3a1 antibody
      • ALDHIII antibody
      • dimeric NADP-preferring antibody
      • MGC10406 antibody
      • Stomach aldehyde dehydrogenase antibody
      • Tumor associated aldehyde dehydrogenase tumor ALDH antibody
      see all

    Images

    • Western blot - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Western blot - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694) at 1/400 dilution + A549 (human lung carcinoma cell line) cell lysate

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human cervix tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human skin tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human esophagus tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human bladder tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab227694? Please let us know so that we can cite the reference in this datasheet.

    ab227694 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com