RecombinantRabMAb

Recombinant Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-ALDH3A1 antibody [SP298] - C-terminal
    See all ALDH3A1 primary antibodies

  • Description

    Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ALDH3A1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P30838

  • Positive control

    • WB: A549 cell lysate. IHC-P: Human bladder, esophagus, skin, cervix, tonsil, stomach, stomach adenocarcinoma, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 50 kDa.
IHC-P 1/100.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.

Target

  • Function

    ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

  • Tissue specificity

    High levels in stomach, esophagus and lung; low level in the liver and kidney.

  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Acetaldehyde dehydrogenase 3 antibody
      • AHD 4 antibody
      • AHD C antibody
      • AHD4 antibody
      • AHDC antibody
      • AL3A1_HUMAN antibody
      • Aldehyde dehydrogenase 3 antibody
      • Aldehyde dehydrogenase 3 family member A1 antibody
      • Aldehyde dehydrogenase 3A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase class 3 antibody
      • Aldehyde dehydrogenase dimeric NADP preferring antibody
      • Aldehyde dehydrogenase family 3 member A1 antibody
      • Aldehyde dehydrogenase family 3 subfamily A1 antibody
      • Aldehyde dehydrogenase family 3, subfamily A, member 1 antibody
      • Aldehyde dehydrogenase isozyme 3 antibody
      • Aldehyde dehydrogenase type III antibody
      • ALDH 3 antibody
      • ALDH 3A1 antibody
      • ALDH III antibody
      • ALDH, stomach type antibody
      • ALDH3 A1 antibody
      • ALDH3 antibody
      • ALDH3A 1 antibody
      • Aldh3a1 antibody
      • ALDHIII antibody
      • dimeric NADP-preferring antibody
      • MGC10406 antibody
      • Stomach aldehyde dehydrogenase antibody
      • Tumor associated aldehyde dehydrogenase tumor ALDH antibody
      see all

    Images

    • Western blot - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Western blot - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694) at 1/400 dilution + A549 (human lung carcinoma cell line) cell lysate

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human cervix tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human skin tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human esophagus tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human bladder tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

    • Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
      Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab227694? Please let us know so that we can cite the reference in this datasheet.

    ab227694 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com