Recombinant Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ALDH3A1 antibody [SP298] - C-terminal
See all ALDH3A1 primary antibodies -
Description
Rabbit monoclonal [SP298] to ALDH3A1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: A549 cell lysate. IHC-P: Human bladder, esophagus, skin, cervix, tonsil, stomach, stomach adenocarcinoma, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP298 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/400. Predicted molecular weight: 50 kDa.
|
|
IHC-P |
1/100.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
Notes |
---|
WB
1/400. Predicted molecular weight: 50 kDa. |
IHC-P
1/100. Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
Target
-
Function
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. -
Tissue specificity
High levels in stomach, esophagus and lung; low level in the liver and kidney. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 218 Human
- Omim: 100660 Human
- SwissProt: P30838 Human
- Unigene: 531682 Human
-
Alternative names
- Acetaldehyde dehydrogenase 3 antibody
- AHD 4 antibody
- AHD C antibody
see all
Images
-
Anti-ALDH3A1 antibody [SP298] - C-terminal (ab227694) at 1/400 dilution + A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 50 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cervix tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human skin tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human esophagus tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human bladder tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human stomach tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab227694 has not yet been referenced specifically in any publications.