Anti-ALDH3A2 antibody (ab113111)
Key features and details
- Rabbit polyclonal to ALDH3A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ALDH3A2 antibody -
Description
Rabbit polyclonal to ALDH3A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Recombinant fragment, corresponding to amino acids 166-400 of Human ALDH3A2 (BC002430).
-
Positive control
- 293T cell lysate, A549 cell lysate, Human fetal testis lysate, Human fetal skeletal muscle tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab113111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). | |
| IHC-P | 1/100 - 1/500. |
Target
-
Relevance
Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. -
Cellular localization
Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. -
Database links
- Entrez Gene: 224 Human
- Entrez Gene: 11671 Mouse
- Entrez Gene: 65183 Rat
- Omim: 609523 Human
- SwissProt: P51648 Human
- SwissProt: P47740 Mouse
- SwissProt: P30839 Rat
-
Alternative names
- Aldehyde dehydrogenase antibody
- Aldehyde dehydrogenase 10 antibody
- Aldehyde dehydrogenase 3 family member A2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A2 antibody (ab113111)ab113111, at 1/100 dilution, staining ALDH3A2 in formalin-fixed, paraffin-embedded Human Fetal skeletal muscle by Immunohistochemistry.
-
Western blot - Anti-ALDH3A2 antibody (ab113111)All lanes : Anti-ALDH3A2 antibody (ab113111) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Human fetal testis lysate
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A2 antibody (ab113111)This image is courtesy of an Abreview from Zachary Yu-Ching Lin.IHC-P image of ALDH3A2 staining with ab113111 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab113111 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Protocols
References (3)
ab113111 has been referenced in 3 publications.
- Sarret C et al. Deleterious mutations in ALDH1L2 suggest a novel cause for neuro-ichthyotic syndrome. NPJ Genom Med 4:17 (2019). PubMed: 31341639
- Kang JH et al. Aldehyde dehydrogenase is used by cancer cells for energy metabolism. Exp Mol Med 48:e272 (2016). WB ; Human . PubMed: 27885254
- Xiao N et al. A novel aldehyde dehydrogenase-3 activator (Alda-89) protects submandibular gland function from irradiation without accelerating tumor growth. Clin Cancer Res 19:4455-64 (2013). PubMed: 23812668
