Overview

  • Product name

    Anti-ALDH4A1/P5CDH antibody
    See all ALDH4A1/P5CDH primary antibodies
  • Description

    Rabbit polyclonal to ALDH4A1/P5CDH
  • Host species

    Rabbit
  • Specificity

    ab196612 detects endogenous level of total ALDH4A1/P5CDH protein.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human ALDH4A1/P5CDH (C terminal). The exact sequence is proprietary.
    Database link: P30038

  • Positive control

    • HepG2, K562, A469 and BT474 cell lysates; mouse skeletal muscle, intestine and kidney lysates. Human breast cancer, Human thyroid cancer and mouse lung tissues. A549 cells.
  • General notes

     This product was previously labelled as ALDH4A1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab196612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/50 - 1/200. Predicted molecular weight: 62 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
  • Tissue specificity

    Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
  • Pathway

    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
  • Involvement in disease

    Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • AL4A1_HUMAN antibody
    • aldehyde dehydrogenase 4 antibody
    • aldehyde dehydrogenase 4 family, member A1 antibody
    • Aldehyde dehydrogenase family 4 member A1 antibody
    • Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
    • ALDH4 antibody
    • aldh4a1 antibody
    • Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
    • Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
    • L-glutamate gamma-semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase antibody
    • P5C dehydrogenase antibody
    • P5CD antibody
    • P5CDh antibody
    • P5CDhL antibody
    • P5CDhS antibody
    • Pyrroline-5-carboxylate dehydrogenase antibody
    • RP11 128M10.1 antibody
    see all

Images

  • All lanes : Anti-ALDH4A1/P5CDH antibody (ab196612) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : BT474 cell lysate
    Lane 5 : mouse skeletal muscle lysate
    Lane 6 : mouse intestine lysate
    Lane 7 : mouse kidney lysate

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ALDH4A1/P5CDH using ab196612 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ALDH4A1/P5CDH using ab196612 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling ALDH4A1/P5CDH using ab196612 at a 1/200 dilution.

  • Immunofluorescence analysis of A549 cells, labeling ALDH4A1/P5CDH using ab196612 at a 1/50 dilution.

References

ab196612 has not yet been referenced specifically in any publications.

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