Recombinant Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free (ab250807)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-ALDH4A1/P5CDH antibody [EPR14287] - BSA and Azide free
See all ALDH4A1/P5CDH primary antibodies -
Description
Rabbit monoclonal [EPR14287] to ALDH4A1/P5CDH - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250807 is the carrier-free version of ab185208.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14287 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
Target
-
Function
Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. -
Tissue specificity
Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. -
Pathway
Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. -
Involvement in disease
Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 8659 Human
- Entrez Gene: 212647 Mouse
- Entrez Gene: 641316 Rat
- Omim: 606811 Human
- SwissProt: P30038 Human
- SwissProt: Q8CHT0 Mouse
- SwissProt: P0C2X9 Rat
- Unigene: 77448 Human
see all -
Alternative names
- AL4A1_HUMAN antibody
- aldehyde dehydrogenase 4 antibody
- aldehyde dehydrogenase 4 family, member A1 antibody
see all
Images
-
Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/1000 dilution + Human fetal heart at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDaThis data was developed using ab185208, the same antibody clone in a different buffer formulation.
-
This data was developed using ab185208, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
This data was developed using ab185208, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of -20? acetone fixed HeLa cells labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution (red). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi (blue).
-
This data was developed using ab185208, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling ALDH4A1/P5CDH with ab185208 at 1/160 dilution (red), or a rabbit IgG isotype control (green), followed by secondary goat anti rabbit IgG (FITC) at 1/150 dilution.
-
Anti-ALDH4A1/P5CDH antibody [EPR14287] (ab185208) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDaThis data was developed using ab185208, the same antibody clone in a different buffer formulation.
-
This data was developed using ab185208, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ALDH4A1/P5CDH with ab185208 at 1/250 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250807 has not yet been referenced specifically in any publications.