Overview

  • Product name

    Anti-ALDH4A1/P5CDH antibody [EPR14288(B)]
    See all ALDH4A1/P5CDH primary antibodies
  • Description

    Rabbit monoclonal [EPR14288(B)] to ALDH4A1/P5CDH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human ALDH4A1/P5CDH aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P30038

  • Positive control

    • HepG2, HeLa and K562 cell lysates; Human fetal heart and liver lysates; Human liver and kidney tissues; HepG2 cells.
  • General notes

     

     

     This product was previously labelled as ALDH4A1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/1000.
IP 1/40 - 1/60.

Target

  • Function

    Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
  • Tissue specificity

    Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
  • Pathway

    Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
  • Involvement in disease

    Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2) [MIM:239510]. HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • AL4A1_HUMAN antibody
    • aldehyde dehydrogenase 4 antibody
    • aldehyde dehydrogenase 4 family, member A1 antibody
    • Aldehyde dehydrogenase family 4 member A1 antibody
    • Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
    • ALDH4 antibody
    • aldh4a1 antibody
    • Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
    • Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
    • L-glutamate gamma-semialdehyde dehydrogenase antibody
    • mitochondrial antibody
    • mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase antibody
    • P5C dehydrogenase antibody
    • P5CD antibody
    • P5CDh antibody
    • P5CDhL antibody
    • P5CDhS antibody
    • Pyrroline-5-carboxylate dehydrogenase antibody
    • RP11 128M10.1 antibody
    see all

Images

  • All lanes : Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Anti-ALDH4A1/P5CDH antibody [EPR14288(B)] (ab181256) at 1/20000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling ALDH4A1/P5CDH with ab181256 at a 1/250 dilution. Counter stained hematoxylin.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling ALDH4A1/P5CDH with ab181256 at a 1/1000 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200 dilution. In blue DAPI staining.

  • Western blot analysis on Immunoprecipitation from HepG2 cell lysate; showing ALDH4A1/P5CDH, using ab181256 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab181256 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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