Anti-ALDH5A1/SSADH antibody (ab155493)
Key features and details
- Rabbit polyclonal to ALDH5A1/SSADH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH5A1/SSADH antibody
See all ALDH5A1/SSADH primary antibodies -
Description
Rabbit polyclonal to ALDH5A1/SSADH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human ALDH5A1/SSADH aa 85-324.
Database link: P51649 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji, Mouse brain whole cell lysate; A431 cells; A549 xenograft
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General notes
Keep as a concentrated solution.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/1000. Suggested antigen
retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). -
Tissue specificity
Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta. -
Pathway
Amino-acid degradation; 4-aminobutanoate degradation. -
Involvement in disease
Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 532724 Cow
- Entrez Gene: 7915 Human
- Entrez Gene: 214579 Mouse
- Omim: 610045 Human
- SwissProt: P51649 Human
- SwissProt: Q8BWF0 Mouse
- Unigene: 371723 Human
- Unigene: 393311 Mouse
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Alternative names
- Aldedehyde dehydrogenase 5 family antibody
- Aldehyde dehydrogenase 5 family member A1 antibody
- Aldehyde dehydrogenase 5A1 antibody
see all
Images
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Anti-ALDH5A1/SSADH antibody (ab155493) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Paraffin-embedded mouse liver tissue labeling ALDH5A1/SSADH using ab155493 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Anti-ALDH5A1/SSADH antibody (ab155493) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed A431 cells labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution. Lower panel co-stained with Hoescht 33342.
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Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155493 has not yet been referenced specifically in any publications.