Overview

  • Product name

    Anti-ALDH5A1/SSADH antibody
    See all ALDH5A1/SSADH primary antibodies
  • Description

    Rabbit polyclonal to ALDH5A1/SSADH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human ALDH5A1/SSADH aa 85-324.
    Database link: P51649

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji, Mouse brain whole cell lysate; A431 cells; A549 xenograft
  • General notes

    Keep as a concentrated solution.

     This product was previously labelled as ALDH5A1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
  • Tissue specificity

    Brain, pancreas, heart, liver, skeletal muscle and kidney. Lower in placenta.
  • Pathway

    Amino-acid degradation; 4-aminobutanoate degradation.
  • Involvement in disease

    Defects in ALDH5A1 are the cause of succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) [MIM:271980]. SSADH deficiency is a rare inborn error in the metabolism of 4-aminobutyric acid (GABA) which leads to accumulation of 4-hydroxybutyric acid in physiologic fluids of patients. The disease is characterized by severe ataxia and by mildly retarded psychomotor development.
  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aldedehyde dehydrogenase 5 family antibody
    • Aldehyde dehydrogenase 5 family member A1 antibody
    • Aldehyde dehydrogenase 5A1 antibody
    • Aldehyde dehydrogenase family 5 member A1 antibody
    • ALDH5A 1 antibody
    • Aldh5a1 antibody
    • mitochondrial antibody
    • Mitochondrial succinate semialdehyde dehydrogenase antibody
    • NAD(+) dependent succinic semialdehyde dehydrogenase antibody
    • NAD(+)-dependent succinic semialdehyde dehydrogenase antibody
    • SSADH antibody
    • SSDH antibody
    • SSDH_HUMAN antibody
    • Succinate semialdehyde dehydrogenase antibody
    • Succinate-semialdehyde dehydrogenase antibody
    see all

Images

  • Anti-ALDH5A1/SSADH antibody (ab155493) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Paraffin-embedded mouse liver tissue labeling ALDH5A1/SSADH using ab155493 at 1/500 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.

  • Anti-ALDH5A1/SSADH antibody (ab155493) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution. Lower panel co-stained with Hoescht 33342.

  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling ALDH5A1/SSADH with ab155493 at 1/500 dilution.

References

ab155493 has not yet been referenced specifically in any publications.

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