Anti-ALDH6A1 antibody (ab228584)
Key features and details
- Rabbit polyclonal to ALDH6A1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH6A1 antibody -
Description
Rabbit polyclonal to ALDH6A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment within Human ALDH6A1 (internal sequence). The exact sequence is proprietary.
Database link: Q02252 -
Positive control
- WB: Mouse liver tissue whole cell lysate; HepG2 whole cell lysate. IHC-P: Mouse stomach tissue; human liver carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA. -
Involvement in disease
Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDH deficiency) [MIM:603178]. This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 327692 Cow
- Entrez Gene: 4329 Human
- Entrez Gene: 104776 Mouse
- Entrez Gene: 81708 Rat
- Omim: 603178 Human
- SwissProt: Q07536 Cow
- SwissProt: Q02252 Human
- SwissProt: Q9EQ20 Mouse
see all -
Alternative names
- 1110038I05Rik antibody
- AI314632 antibody
- Aldehyde dehydrogenase 6 family member A1 antibody
see all
Images
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Paraffin-embedded mouse stomach tissue stained for ALDH6A1 with ab228584 at 1/500 dilution in immunohistochemical analysis.
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Anti-ALDH6A1 antibody (ab228584) at 1/1000 dilution + Mouse liver tissue whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa7.5% SDS-PAGE
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Anti-ALDH6A1 antibody (ab228584) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 58 kDa7.5% SDS-PAGE
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Paraffin-embedded human liver carcinoma tissue stained for ALDH6A1 with ab228584 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228584 has not yet been referenced specifically in any publications.