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    aldh7a1-antibody-ab154218.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-ALDH7A1 antibody (ab154218)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
  • Western blot - Anti-ALDH7A1 antibody (ab154218)

Key features and details

  • Rabbit polyclonal to ALDH7A1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ALDH7A1 antibody
    See all ALDH7A1 primary antibodies
  • Description

    Rabbit polyclonal to ALDH7A1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 70-481 of Human ALDH7A1 (UniProt: P49419).

  • Positive control

    • HepG2 whole cell lysate; Human Mahlarvu xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Target information above from: UniProt accession P49419 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 507477 Cow
    • Entrez Gene: 501 Human
    • Entrez Gene: 110695 Mouse
    • Entrez Gene: 549131 Xenopus tropicalis
    • Omim: 107323 Human
    • SwissProt: Q2KJC9 Cow
    • SwissProt: P49419 Human
    • SwissProt: Q9DBF1 Mouse
    • Unigene: 483239 Human
    • Unigene: 30250 Mouse
    see all
  • Alternative names

    • 26g turgor protein homolog antibody
    • AL7A1_HUMAN antibody
    • Aldehyde dehydrogenase 7 A1 antibody
    • Aldehyde dehydrogenase 7 family, member A1 antibody
    • Aldehyde dehydrogenase family 7 member A1 antibody
    • ALDH7A1 antibody
    • Alpha AASA dehydrogenase antibody
    • Alpha aminoadipic semialdehyde dehydrogenase antibody
    • Alpha-AASA dehydrogenase antibody
    • Alpha-aminoadipic semialdehyde dehydrogenase antibody
    • Antiquitin 1 antibody
    • Antiquitin antibody
    • Antiquitin-1 antibody
    • ATQ1 antibody
    • Betaine aldehyde dehydrogenase antibody
    • Delta1 piperideine 6 carboxylate dehydrogenease antibody
    • Delta1-piperideine-6-carboxylate dehydrogenase antibody
    • EPD antibody
    • P6c dehydrogenase antibody
    • PDE antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
    Immunohistochemical analysis of paraffin-embedded Human Mahlarvu xenograft tissue, labeling ALDH7A1 with ab154218 at 1/500 dilution.
  • Western blot - Anti-ALDH7A1 antibody (ab154218)
    Western blot - Anti-ALDH7A1 antibody (ab154218)
    Anti-ALDH7A1 antibody (ab154218) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154218? Please let us know so that we can cite the reference in this datasheet.

ab154218 has been referenced in 1 publication.

  • Shahid M  et al. Centromere protein F (CENPF), a microtubule binding protein, modulates cancer metabolism by regulating pyruvate kinase M2 phosphorylation signaling. Cell Cycle N/A:N/A (2018). PubMed: 30526248

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