Anti-ALDH7A1 antibody (ab154218)
Key features and details
- Rabbit polyclonal to ALDH7A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ALDH7A1 antibody
See all ALDH7A1 primary antibodies -
Description
Rabbit polyclonal to ALDH7A1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 70-481 of Human ALDH7A1 (UniProt: P49419).
-
Positive control
- HepG2 whole cell lysate; Human Mahlarvu xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
All applications |
Mouse
Cow
Xenopus tropicalis
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 507477 Cow
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Entrez Gene: 549131 Xenopus tropicalis
- Omim: 107323 Human
- SwissProt: Q2KJC9 Cow
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
see all -
Alternative names
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)Immunohistochemical analysis of paraffin-embedded Human Mahlarvu xenograft tissue, labeling ALDH7A1 with ab154218 at 1/500 dilution.
-
Anti-ALDH7A1 antibody (ab154218) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab154218 has been referenced in 1 publication.
- Shahid M et al. Centromere protein F (CENPF), a microtubule binding protein, modulates cancer metabolism by regulating pyruvate kinase M2 phosphorylation signaling. Cell Cycle N/A:N/A (2018). PubMed: 30526248