Anti-ALDH7A1 antibody (ab154218)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
  • Western blot - Anti-ALDH7A1 antibody (ab154218)

Key features and details

  • Rabbit polyclonal to ALDH7A1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ALDH7A1 antibody
    See all ALDH7A1 primary antibodies

  • Description

    Rabbit polyclonal to ALDH7A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 70-481 of Human ALDH7A1 (UniProt: P49419).

  • Positive control

    • HepG2 whole cell lysate; Human Mahlarvu xenograft tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab154218)
    Immunohistochemical analysis of paraffin-embedded Human Mahlarvu xenograft tissue, labeling ALDH7A1 with ab154218 at 1/500 dilution.
  • Western blot - Anti-ALDH7A1 antibody (ab154218)
    Western blot - Anti-ALDH7A1 antibody (ab154218)
    Anti-ALDH7A1 antibody (ab154218) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE

References (1)

Publishing research using ab154218? Please let us know so that we can cite the reference in this datasheet.

ab154218 has been referenced in 1 publication.

  • Shahid M  et al. Centromere protein F (CENPF), a microtubule binding protein, modulates cancer metabolism by regulating pyruvate kinase M2 phosphorylation signaling. Cell Cycle N/A:N/A (2018). PubMed: 30526248

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