Anti-ALDH7A1 antibody (ab154264)
Key features and details
- Rabbit polyclonal to ALDH7A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH7A1 antibody
See all ALDH7A1 primary antibodies -
Description
Rabbit polyclonal to ALDH7A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 261-479 of Human ALDH7A1.
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Positive control
- A431, H1299, HeLa, HepG2 and mouse liver lysates; HeLa cells; MCF7 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 507477 Cow
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Omim: 107323 Human
- SwissProt: Q2KJC9 Cow
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- Unigene: 483239 Human
see all -
Alternative names
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
Images
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Anti-ALDH7A1 antibody (ab154264) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Anti-ALDH7A1 antibody (ab154264) at 1/10000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ALDH7A1 with ab154264 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labeling ALDH7A1 with ab154264 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154264 has been referenced in 1 publication.
- Struebing FL et al. Genomic loci modulating retinal ganglion cell death following elevated IOP in the mouse. Exp Eye Res 169:61-67 (2018). IHC-Fr ; Mouse . PubMed: 29421330