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  1. Link

    aldh7a1-antibody-ep1934y-ab68192.pdf

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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

  • Datasheet
  • SDS
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Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
  • Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1934Y] to ALDH7A1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-ALDH7A1 antibody [EP1934Y]
    See all ALDH7A1 primary antibodies
  • Description

    Rabbit monoclonal [EP1934Y] to ALDH7A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ALDH7A1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Lysates from: HepG2 cells, HeLa cells, SW480 cells and SH-SY5Y cells. IHC-P: Human liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1934Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-ALDH7A1 antibody [EP1934Y] - BSA and Azide free (ab236000)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Target information above from: UniProt accession P49419 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 501 Human
    • Entrez Gene: 110695 Mouse
    • Omim: 107323 Human
    • SwissProt: P49419 Human
    • SwissProt: Q9DBF1 Mouse
    • Unigene: 483239 Human
    • Unigene: 30250 Mouse
    • Alternative names

      • 26g turgor protein homolog antibody
      • AL7A1_HUMAN antibody
      • Aldehyde dehydrogenase 7 A1 antibody
      • Aldehyde dehydrogenase 7 family, member A1 antibody
      • Aldehyde dehydrogenase family 7 member A1 antibody
      • ALDH7A1 antibody
      • Alpha AASA dehydrogenase antibody
      • Alpha aminoadipic semialdehyde dehydrogenase antibody
      • Alpha-AASA dehydrogenase antibody
      • Alpha-aminoadipic semialdehyde dehydrogenase antibody
      • Antiquitin 1 antibody
      • Antiquitin antibody
      • Antiquitin-1 antibody
      • ATQ1 antibody
      • Betaine aldehyde dehydrogenase antibody
      • Delta1 piperideine 6 carboxylate dehydrogenease antibody
      • Delta1-piperideine-6-carboxylate dehydrogenase antibody
      • EPD antibody
      • P6c dehydrogenase antibody
      • PDE antibody
      see all

    Images

    • Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
      Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
      All lanes : Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

      Human liver tissue stained by ab68192 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
      Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab68192? Please let us know so that we can cite the reference in this datasheet.

    ab68192 has been referenced in 2 publications.

    • Etienne J  et al. Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients. J Cachexia Sarcopenia Muscle 11:1047-1069 (2020). PubMed: 32157826
    • Meyer C  et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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