Recombinant Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
![Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)](https://www.abcam.com/ps/products/68/ab68192/Images/ab68192-82388-anti-aldh7a1-antibody-ep1934y-western-blot.jpg "Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1934Y] to ALDH7A1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ALDH7A1 antibody [EP1934Y]
See all ALDH7A1 primary antibodies -
Description
Rabbit monoclonal [EP1934Y] to ALDH7A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human ALDH7A1 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Lysates from: HepG2 cells, HeLa cells, SW480 cells and SH-SY5Y cells. IHC-P: Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1934Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Omim: 107323 Human
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- Unigene: 483239 Human
- Unigene: 30250 Mouse
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Alternative names
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
Images
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Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)All lanes : Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)](https://www.abcam.com/ps/products/68/ab68192/Images/ab68192-82387-anti-aldh7a1-antibody-ep1934y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)Human liver tissue stained by ab68192 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ALDH7A1 antibody [EP1934Y] (ab68192)](https://www.abcam.com/ps/products/68/ab68192/Images/ab68192-3-benefits-of-recombinant-antibodies.png)
Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab68192 has been referenced in 2 publications.
- Etienne J et al. Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients. J Cachexia Sarcopenia Muscle 11:1047-1069 (2020). PubMed: 32157826
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924