Recombinant Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-ALDH7A1 antibody [EP1934Y]
See all ALDH7A1 primary antibodies -
Description
Rabbit monoclonal [EP1934Y] to ALDH7A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ALDH7A1 aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- WB: Lysates from: HepG2 cells, HeLa cells, SW480 cells and SH-SY5Y cells. IHC-P: Human liver tissue.
-
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1934Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab68192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). | |
| IP | 1/50. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 501 Human
- Entrez Gene: 110695 Mouse
- Omim: 107323 Human
- SwissProt: P49419 Human
- SwissProt: Q9DBF1 Mouse
- Unigene: 483239 Human
- Unigene: 30250 Mouse
-
Alternative names
- 26g turgor protein homolog antibody
- AL7A1_HUMAN antibody
- Aldehyde dehydrogenase 7 A1 antibody
see all
Images
-
![Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)](https://www.abcam.com/ps/products/68/ab68192/Images/ab68192-82388-anti-aldh7a1-antibody-ep1934y-western-blot.jpg)
Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)All lanes : Anti-ALDH7A1 antibody [EP1934Y] (ab68192)
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)](https://www.abcam.com/ps/products/68/ab68192/Images/ab68192-82387-anti-aldh7a1-antibody-ep1934y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)Human liver tissue stained by ab68192 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). Read more (PubMed: 29429924) »
