Product nameALDOA positive control ChIP primer pair
See all Aldolase primary antibodies
DescriptionALDOA positive control ChIP primer pair
Tested applicationsSuitable for: ChIPmore details
Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K4 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab269260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab32356 (red), and 20µl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269260 has not yet been referenced specifically in any publications.