Overview

  • Product name
    Aldolase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type
    Enzyme activity
  • Sensitivity
    < 100 µU
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Aldolase Activity Assay (Colorimetric) (ab196994), aldolase converts fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone, and through a series of reactions, reduces a nearly colorless probe to a colored product with absorbance at 450 nm.

    This assay kit is simple, sensitive and high-throughput adaptable and it can be used to measure aldolase activity in various cells and tissues as well as to analyze glycolysis and glucogenesis pathways.

  • Notes

    Aldolase (Fructose-Bisphosphate Aldolase: EC 4.1.2.13) is an important enzyme for both glycolysis and gluconeogenesis. It catalyzes the reversible reaction of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate & dihydroxyacetone.

    There are 2 classes of Aldolase - class l: found in animal and plant tissues and class II: found in prokaryotes and lower eukaryotes. Class I Aldolase has 3 isozymes- Type A: found in muscle and red blood cells, Type B: found in liver and kidney and Type C: found in brain. Aldolase A deficiency leads to myopathy & hemolytic anemia. Muscle disease and liver injury can also cause increased serum aldolase.

    Accurate detection of aldolase activity is valuable for diagnostic and mechanistic studies.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Aldolase Assay Buffer 1 x 25ml
    Aldolase Developer 1 vial
    Aldolase Enzyme Mix 1 vial
    Aldolase Substrate 1 vial
    NADH Standard Yellow 1 vial
    Positive Control 1 vial
  • Function
    Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease
    Glycogen storage disease 12
  • Sequence similarities
    Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • Alternative names
    • ALDOA
    • ALDOA_HUMAN
    • Fructose-bisphosphate aldolase A
    • Lung cancer antigen NY-LU-1
    • Muscle-type aldolase
    see all

Images

  • Typical NADH Standard Curve.

  • Aldolase activity in rat kidney and muscle, HeLa and 3T3 cells. Data obtained performing assay as described in assay protocol.

  • Relative aldolase activity in lysates prepared from rat kidney (14.2 μg), rat muscle (10.64 μg), 3T3 cells (9.97 μg) and HeLa cells (11.35 μg).

Protocols

References

This product has been referenced in:
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