Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9724(B)] to Aldolase
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Pig
Product nameAnti-Aldolase antibody [EPR9724(B)]
See all Aldolase primary antibodies
DescriptionRabbit monoclonal [EPR9724(B)] to Aldolase
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human, Pig
Synthetic peptide within Human Aldolase aa 1-100. The exact sequence is proprietary.
Database link: P04075
- WB: A549 and HeLa whole cell lysate (ab150035); human fetal brain, fetal liver and skeletal muscle, mouse brain, rat brain tissue lysates. Flow Cyt: MCF7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 0.02 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lane 4 : Pig heart lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilution (Unpurifed)
Lane 1 : A549 lysate
Lane 2 : Fetal liver lysate
Lane 3 : HeLa lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
ab169544 has been referenced in 7 publications.
- Shen Y et al. LncRNA KCNQ1OT1 sponges miR-34c-5p to promote osteosarcoma growth via ALDOA enhanced aerobic glycolysis. Cell Death Dis 11:278 (2020). PubMed: 32332718
- Li X et al. Fructose-Bisphosphate Aldolase A Regulates Hypoxic Adaptation in Hepatocellular Carcinoma and Involved with Tumor Malignancy. Dig Dis Sci N/A:N/A (2019). PubMed: 31041640
- Xu Z et al. miR-122-5p Inhibits the Proliferation, Invasion and Growth of Bile Duct Carcinoma Cells by Targeting ALDOA. Cell Physiol Biochem 48:2596-2606 (2018). PubMed: 30121648
- Kawai K et al. Fructose-bisphosphate aldolase A is a key regulator of hypoxic adaptation in colorectal cancer cells and involved in treatment resistance and poor prognosis. Int J Oncol 50:525-534 (2017). PubMed: 28000858
- Zeng Y et al. G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers. Oncotarget 7:74526-74536 (2016). WB . PubMed: 27793029
- Godar RJ et al. Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury. Autophagy 11:1537-60 (2015). WB ; Mouse . PubMed: 26103523
- Shi S et al. Metabolic tumor burden is associated with major oncogenomic alterations and serum tumor markers in patients with resected pancreatic cancer. Cancer Lett 360:227-33 (2015). WB ; Human . PubMed: 25687883