WBmore details Unsuitable for:
ICC/IF or IHC-P
Mouse, Rat, Human, Pig
Synthetic peptide within Human Aldolase aa 1-100. The exact sequence is proprietary. Database link: P04075
WB: A549 and HeLa cell lysates; human fetal brain, fetal liver and skeletal muscle, mouse brain, rat brain tissue lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Belongs to the class I fructose-bisphosphate aldolase family.
Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Aldolase antibody [EPR9724(B)] (ab169544)
All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilution (Unpurifed)
Lane 1 : A549 lysate Lane 2 : Fetal liver lysate Lane 3 : HeLa lysate Lane 4 : Fetal brain lysate Lane 5 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Zeng Y et al. G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers. Oncotarget7:74526-74536 (2016).
Read more (PubMed: 27793029) »
Godar RJ et al. Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury. Autophagy11:1537-60 (2015).
Read more (PubMed: 26103523) »