Recombinant
RabMAb

Anti-Aldolase antibody [EPR9724(B)] (ab169544)

Overview

  • Product name
    Anti-Aldolase antibody [EPR9724(B)]
    See all Aldolase primary antibodies
  • Description
    Rabbit monoclonal [EPR9724(B)] to Aldolase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human Aldolase aa 1-100. The exact sequence is proprietary.
    Database link: P04075

  • Positive control
    • WB: A549 and HeLa cell lysates; human fetal brain, fetal liver and skeletal muscle, mouse brain, rat brain tissue lysates. FC: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
    • Pathway
      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in disease
      Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
    • Sequence similarities
      Belongs to the class I fructose-bisphosphate aldolase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDA antibody
      • Aldo1 antibody
      • ALDOA antibody
      • ALDOA_HUMAN antibody
      • Aldolase 1 antibody
      • Aldolase A antibody
      • Aldolase A fructose bisphosphatase antibody
      • Aldolase A fructose bisphosphate antibody
      • Aldolase, fructose-bisphosphate A antibody
      • Epididymis secretory sperm binding protein Li 87p antibody
      • FRUCTOALDOLASE A antibody
      • Fructose 1 6 bisphosphate triosephosphate lyase antibody
      • Fructose bisphosphate aldolase A antibody
      • Fructose bisphosphate aldolase antibody
      • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
      • Fructose-bisphosphate aldolase A antibody
      • Fructose-bisphosphate aldolase A Muscle-type antibody
      • GSD12 antibody
      • HEL S 87p antibody
      • Lung cancer antigen NY LU 1 antibody
      • Lung cancer antigen NY-LU-1 antibody
      • MGC10942 antibody
      • MGC17716 antibody
      • MGC17767 antibody
      • Muscle type aldolase antibody
      • Muscle-type aldolase antibody
      • RNALDOG5 antibody
      see all

    Images

    • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 0.02 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates
      Lane 4 : Pig heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilution (Unpurifed)

      Lane 1 : A549 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HeLa lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    References

    This product has been referenced in:
    • Zeng Y  et al. G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers. Oncotarget 7:74526-74536 (2016). WB . Read more (PubMed: 27793029) »
    • Godar RJ  et al. Repetitive stimulation of autophagy-lysosome machinery by intermittent fasting preconditions the myocardium to ischemia-reperfusion injury. Autophagy 11:1537-60 (2015). WB ; Mouse . Read more (PubMed: 26103523) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 29 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Tissue lysate - whole (CNS tissue)
    Specification
    CNS tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2014

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Mouse Tissue lysate - whole (Brain tissue)
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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