Recombinant
RabMAb

Recombinant Anti-Aldolase antibody [EPR9724(B)] (Alexa Fluor® 488) (ab200771)

Overview

  • Product name
    Anti-Aldolase antibody [EPR9724(B)] (Alexa Fluor® 488)
    See all Aldolase primary antibodies
  • Description
    Rabbit monoclonal [EPR9724(B)] to Aldolase (Alexa Fluor® 488)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Aldolase.
    Database link: P04075

  • Positive control
    • Flow Cyt: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Target

  • Function
    Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease
    Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
  • Sequence similarities
    Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALDA antibody
    • Aldo1 antibody
    • ALDOA antibody
    • ALDOA_HUMAN antibody
    • Aldolase 1 antibody
    • Aldolase A antibody
    • Aldolase A fructose bisphosphatase antibody
    • Aldolase A fructose bisphosphate antibody
    • Aldolase, fructose-bisphosphate A antibody
    • Epididymis secretory sperm binding protein Li 87p antibody
    • FRUCTOALDOLASE A antibody
    • Fructose 1 6 bisphosphate triosephosphate lyase antibody
    • Fructose bisphosphate aldolase A antibody
    • Fructose bisphosphate aldolase antibody
    • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
    • Fructose-bisphosphate aldolase A antibody
    • Fructose-bisphosphate aldolase A Muscle-type antibody
    • GSD12 antibody
    • HEL S 87p antibody
    • Lung cancer antigen NY LU 1 antibody
    • Lung cancer antigen NY-LU-1 antibody
    • MGC10942 antibody
    • MGC17716 antibody
    • MGC17767 antibody
    • Muscle type aldolase antibody
    • Muscle-type aldolase antibody
    • RNALDOG5 antibody
    see all

Images

  • Overlay histogram showing MCF7 cells stained with ab200771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab200771, 1/100 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

ab200771 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200771.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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