Recombinant
RabMAb

Recombinant Anti-Aldolase antibody [EPR9724(B)] - BSA and Azide free (ab231682)

Overview

  • Product name
    Anti-Aldolase antibody [EPR9724(B)] - BSA and Azide free
    See all Aldolase primary antibodies
  • Description
    Rabbit monoclonal [EPR9724(B)] to Aldolase - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aldolase aa 1-100. The exact sequence is proprietary.
    Database link: P04075

  • Positive control
    • IHC-P: Human ovarian carcinoma tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab231682 is a PBS-only buffer format of ab169544. Please refer to ab169544 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab231682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
    • Pathway
      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in disease
      Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
    • Sequence similarities
      Belongs to the class I fructose-bisphosphate aldolase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALDA antibody
      • Aldo1 antibody
      • ALDOA antibody
      • ALDOA_HUMAN antibody
      • Aldolase 1 antibody
      • Aldolase A antibody
      • Aldolase A fructose bisphosphatase antibody
      • Aldolase A fructose bisphosphate antibody
      • Aldolase, fructose-bisphosphate A antibody
      • Epididymis secretory sperm binding protein Li 87p antibody
      • FRUCTOALDOLASE A antibody
      • Fructose 1 6 bisphosphate triosephosphate lyase antibody
      • Fructose bisphosphate aldolase A antibody
      • Fructose bisphosphate aldolase antibody
      • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
      • Fructose-bisphosphate aldolase A antibody
      • Fructose-bisphosphate aldolase A Muscle-type antibody
      • GSD12 antibody
      • HEL S 87p antibody
      • Lung cancer antigen NY LU 1 antibody
      • Lung cancer antigen NY-LU-1 antibody
      • MGC10942 antibody
      • MGC17716 antibody
      • MGC17767 antibody
      • Muscle type aldolase antibody
      • Muscle-type aldolase antibody
      • RNALDOG5 antibody
      see all

    Images

    • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169544).

    References

    ab231682 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab231682.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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