Product nameAnti-Aldolase antibody [EPR9724(B)] (HRP)
See all Aldolase primary antibodies
DescriptionRabbit monoclonal [EPR9724(B)] to Aldolase (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Aldolase.
- WB : A549 and HeLa whole cell lysates as well as Human fetal liver, fetal brain skeletal muscle tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab200774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 39 kDa.|
FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
All lanes : Anti-Aldolase antibody [EPR9724(B)] (HRP) (ab200774) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : Fetal Liver (Human) Normal Tissue)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Fetal Brain (Human) Normal Tissue lysate
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200774 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab200774 has not yet been referenced specifically in any publications.