Product nameAnti-Aldolase antibody (HRP)
See all Aldolase primary antibodies
DescriptionGoat polyclonal to Aldolase (HRP)
Tested applicationsSuitable for: IP, ELISA, WBmore details
Species reactivityReacts with: Rabbit, Human
Predicted to work with: Mouse, Rat
Full length native protein (purified) corresponding to Rabbit Aldolase aa 1-364. Purified from Rabbit Muscle.
MPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTE NTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKS KGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRC VLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLK RCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKYSHEEIA MATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTF SYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAG AAASESLFIS NHAY
Database link: P00883
- A293 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate, 1% BSA
Concentration information loading...
Purification notesIgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab181662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/20000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 39 kDa.|
FunctionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Involvement in diseaseDefects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
Anti-Aldolase antibody (HRP) (ab181662) at 1/1000 dilution + A293 whole cell lysate
Developed using the ECL technique.
Predicted band size: 39 kDa
4-12% tris glycine gradient gel for SDS-PAGE
Immunoprecipitation and Western Blot analysis.
300 µl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are:
1. Purified aldolase
2. 300 µl antiserum with no antigen (negative control)
3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml)
4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml)
Immunoprecipitation was performed with 300 μl of anti-Aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS.
1. Purified Aldolase (1μg)
2. Precipitation negative control (antiserum + No antigen)
3. 300 μl antiserum + 20 μl Aldolase
4. 300 μl antiserum + 80 μl Aldolase
5. 300 μl antiserum + 120 μl Aldolase
6. 300 μl antiserum + 150 μl Aldolase
7. 300 μl antiserum + 300 μl Aldolase
This product has been referenced in:
- Zhang W et al. Effects of Vitamin A on Expressions of Apoptosis Genes Bax and Bcl-2 in Epithelial Cells of Corneal Tissues Induced by Benzalkonium Chloride in Mice with Dry Eye. Med Sci Monit 25:4583-4589 (2019). Read more (PubMed: 31257361) »