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Signal Transduction Metabolism Energy Metabolism
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Anti-Aldolase B antibody [5E2AD2] (ab129728)

  • Datasheet
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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
  • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)
  • Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)

Key features and details

  • Mouse monoclonal [5E2AD2] to Aldolase B
  • Suitable for: IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-Aldolase B antibody [5E2AD2]
    See all Aldolase B primary antibodies
  • Description

    Mouse monoclonal [5E2AD2] to Aldolase B
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    5E2AD2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Aldolase B protein (ab123165)

Applications

Our Abpromise guarantee covers the use of ab129728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for WB.
  • Target

    • Pathway

      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
    • Involvement in disease

      Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
    • Sequence similarities

      Belongs to the class I fructose-bisphosphate aldolase family.
    • Target information above from: UniProt accession P05062 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 229 Human
      • Entrez Gene: 230163 Mouse
      • Entrez Gene: 24190 Rat
      • Omim: 612724 Human
      • SwissProt: P05062 Human
      • SwissProt: Q91Y97 Mouse
      • SwissProt: P00884 Rat
      • Unigene: 530274 Human
      • Unigene: 482116 Mouse
      • Unigene: 98207 Rat
      see all
    • Alternative names

      • ALDB antibody
      • ALDO B antibody
      • ALDO2 antibody
      • ALDOB antibody
      • ALDOB_HUMAN antibody
      • Aldolase 2 antibody
      • Aldolase B antibody
      • Aldolase B fructose bisphosphate antibody
      • Aldolase2 antibody
      • AldolaseB antibody
      • EC 4.1.2.13 antibody
      • Fructose bisphosphate aldolase B antibody
      • Fructose-bisphosphate aldolase B antibody
      • Liver type aldolase antibody
      • Liver-type aldolase antibody
      • MS1077 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
    • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
      Lane 1: Ladder
      Lane 2: Human liver homogenate lysate
      Lane 3: Rat liver homogenate lysate
      Lane 4: Mouse liver homogenate lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.
    • Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)
      Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab129728? Please let us know so that we can cite the reference in this datasheet.

    ab129728 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Has this antibody been tested for western blotting?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 11 2013

    Answer

    The antibody was tested and found to be negative in western blotting.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Dec 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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