Anti-Aldolase B antibody (ab137628)
Key features and details
- Rabbit polyclonal to Aldolase B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Aldolase B antibody
See all Aldolase B primary antibodies -
Description
Rabbit polyclonal to Aldolase B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human Aldolase B aa 84-288.
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Positive control
- 293T, A431, Jurkat, Raji whole cells lysates; mouse liver lysate; methanol-fixed A431 cells; Breast carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 515263 Cow
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: Q3T0S5 Cow
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
see all -
Alternative names
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
Images
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All lanes : Anti-Aldolase B antibody (ab137628) at 1/1000 dilution
Lane 1 : 293T whole cells lysate
Lane 2 : A431 whole cells lysate
Lane 3 : Jurkat whole cells lysate
Lane 4 : Raji whole cells lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS Page -
Immunofluorescence analysis of methanol-fixed A431 cells laebelling Aldolase B using ab137628 antibody at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.
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Anti-Aldolase B antibody (ab137628) at 1/1000 dilution + mouse liver whole cells lysate at 30 µg
Predicted band size: 39 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137628 has not yet been referenced specifically in any publications.