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Signal Transduction Metabolism Energy Metabolism
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Anti-Aldolase B antibody (ab153828)

  • Datasheet
  • SDS
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Western blot - Anti-Aldolase B antibody (ab153828)
  • Western blot - Anti-Aldolase B antibody (ab153828)
  • Western blot - Anti-Aldolase B antibody (ab153828)
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (ab153828)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (ab153828)

Key features and details

  • Rabbit polyclonal to Aldolase B
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Aldolase B antibody
    See all Aldolase B primary antibodies
  • Description

    Rabbit polyclonal to Aldolase B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Aldolase B aa 15-265.
    Database link: P05062

  • Positive control

    • Recombinant Human Aldolase B protein (ab123165) can be used as a positive control in WB. 293T, A431, HeLa, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; Hela cells; Human hepatoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Aldolase B protein (ab123165)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Aldolase B protein (ab123165)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • Involvement in disease

    Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
  • Sequence similarities

    Belongs to the class I fructose-bisphosphate aldolase family.
  • Target information above from: UniProt accession P05062 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 229 Human
    • Entrez Gene: 230163 Mouse
    • Entrez Gene: 24190 Rat
    • Omim: 612724 Human
    • SwissProt: P05062 Human
    • SwissProt: Q91Y97 Mouse
    • SwissProt: P00884 Rat
    • Unigene: 530274 Human
    • Unigene: 482116 Mouse
    • Unigene: 98207 Rat
    see all
  • Alternative names

    • ALDB antibody
    • ALDO B antibody
    • ALDO2 antibody
    • ALDOB antibody
    • ALDOB_HUMAN antibody
    • Aldolase 2 antibody
    • Aldolase B antibody
    • Aldolase B fructose bisphosphate antibody
    • Aldolase2 antibody
    • AldolaseB antibody
    • EC 4.1.2.13 antibody
    • Fructose bisphosphate aldolase B antibody
    • Fructose-bisphosphate aldolase B antibody
    • Liver type aldolase antibody
    • Liver-type aldolase antibody
    • MS1077 antibody
    see all

Images

  • Western blot - Anti-Aldolase B antibody (ab153828)
    Western blot - Anti-Aldolase B antibody (ab153828)
    All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Western blot - Anti-Aldolase B antibody (ab153828)
    Western blot - Anti-Aldolase B antibody (ab153828)
    Anti-Aldolase B antibody (ab153828) at 1/1000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10 % SDS-PAGE
  • Western blot - Anti-Aldolase B antibody (ab153828)
    Western blot - Anti-Aldolase B antibody (ab153828)
    All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10 % SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (ab153828)
    Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (ab153828)

    Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (ab153828)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (ab153828)
    Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab153828? Please let us know so that we can cite the reference in this datasheet.

ab153828 has not yet been referenced specifically in any publications.

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