- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Aldolase B antibody
See all Aldolase B primary antibodies -
DescriptionRabbit polyclonal to Aldolase B
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Human Aldolase B aa 15-265.
Database link: P05062 -
Positive control
- 293T, A431, HeLa, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; Hela cells; Human hepatoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 39 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
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Involvement in diseaseDefects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
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Sequence similaritiesBelongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
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Database links
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
- SwissProt: P00884 Rat
- Unigene: 530274 Human
see all -
Alternative names
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
Images
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Western blot - Anti-Aldolase B antibody (ab153828)All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Western blot - Anti-Aldolase B antibody (ab153828)Anti-Aldolase B antibody (ab153828) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 39 kDa
10 % SDS-PAGE -
Western blot - Anti-Aldolase B antibody (ab153828)All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (ab153828)Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (ab153828)Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.
Protocols
References
ab153828 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"