Anti-Aldolase B antibody (ab153828)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Aldolase B antibody
See all Aldolase B primary antibodies -
Description
Rabbit polyclonal to Aldolase B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Aldolase B aa 15-265.
Database link: P05062 -
Positive control
- Recombinant Human Aldolase B protein (ab123165) can be used as a positive control in WB. 293T, A431, HeLa, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; Hela cells; Human hepatoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 39 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
- SwissProt: P00884 Rat
- Unigene: 530274 Human
see all -
Alternative names
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
Images
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Western blot - Anti-Aldolase B antibody (ab153828)All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Western blot - Anti-Aldolase B antibody (ab153828)Anti-Aldolase B antibody (ab153828) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 39 kDa
10 % SDS-PAGE -
Western blot - Anti-Aldolase B antibody (ab153828)All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (ab153828)Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (ab153828)Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.
Protocols
References
ab153828 has not yet been referenced specifically in any publications.
